β-Amylase Activity Assay Kit
Cat# E-BC-K005-M-96T
Size : 96T
Brand : Elabscience
Contact local distributor :
Phone : +1 850 650 7790
For research use only.
| Detection Principle | The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance. β-amylase was inactivated by the property of amylase not to be heat-resistant, and then the enzyme activity of total amylase and α-amylase is determined. So the activity of β-amylase can be calculated indirectly. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (535-540 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.97 U/g tissue |
| Detection Range | 0.97-34.74 U/g tissu |
| Precision | inter-assay CV: 3.2% | intra-assay CV: 2.3% |
| Sample Volume | 150 μL(tissue homogenate) |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 1% Epipremnum aureum tissue homogenate | 1 |
| 1% Green pepper tissue homogenate | 1 |
| 1% Corn grain tissue homogenate | 1 |
| 1% Daucus carota tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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