Anti-CD19 Antibody-FITC labled

Cat# CFE8502-25

Size : 25Assays

Brand : Cohesion Biosciences

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Phone : +1 850 650 7790

Product Name:Anti-CD19 Antibody-FITC labled
Cat No:CFE8502
Source:Mouse
Reactivity:H
Applications:IF, FC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description:Mouse monoclonal antibody FITC labled to CD19
Immunogen:Native purified human CD19.
Purification:The antibody was purified by affinity chromatography.
Clonality:Monoclonal (clone: CB19)
Conjugation:FITC
Form:Mouse IgG1. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.
Dilution:10 μl / assay
Gene Symbol:CD19
Alternative Names:B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD antigen CD19
Entrez Gene (Human): 930;
SwissProt (Human): P15391;
Directions for Use:1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Storage/Stability:Shipped and store at 4°C for one year. Do not freeze.

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CFE8504-25
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