Mouse anti-Human/Mouse/Rat/Hamster/Ferret/Cow/Pig/Cat/Dog/Sheep/Goat/Horse/Marmoset Monkey Keratin 18 Antibody [Sodium Azide Free]
Anti-Keratin 18 Antibody, Sodium Azide Free
Anti-Keratin 18 Antibody, Sodium Azide Free
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Product Description
Specifications
| Size | 100 µg |
| Species | Human, Mouse, Rat |
| Specificity | Other species not tested. |
| Host Species | Mouse |
| Accession Number | P05783 |
| Gene Id | 3875 |
| Gene Symbols | KRT18, CYK18, PIG46 |
| Protein Name / Synonyms | Keratin, type I cytoskeletal 18 (Cell proliferation-inducing gene 46 protein) (Cytokeratin-18) (CK-18) (Keratin-18) (K18) |
| Immunogen | A cytoskeletal preparation from A431 cells was used as the immunogen for the Keratin 18 antibody. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS, BSA free, sodium azide free |
| Recommended Applications | Western blot: 1-2ug/ml Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 1-2ug/ml Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cytoplasmic |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Storage
Store the Keratin 18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.