BMI1 (NM_005180) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence A DNA sequence encoding human full-length BMI1
Tag	N-His
Predicted MW	36.9 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 100 mM arginine, 10% glycerol
Bioactivity	In vitro kinase assay substrate (PMID: 28270146) Binding assay (competitor) (PMID: 29402932)
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping	Dry Ice
Reference Data
RefSeq	NP_005171
Locus ID	648
UniProt ID	P35226
Cytogenetics	10p12.2
RefSeq Size	3435
RefSeq ORF	978
Synonyms	flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51
Summary	This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. provided by RefSeq, Sep 2015
Protein Categories	Intracellular Proteins
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors

FAQs

Protein FAQs

SDS

SDS for TP760041

Recombinant Protein Resources

Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein

SKU	Description	Size
LC401584	Lyophilized BMI1 HEK293T cell transient overexpression lysate (as WB positive control)	20 ug
LY401584	Transient overexpression lysate of BMI1 polycomb ring finger oncogene (BMI1) (as WB positive control)	100 ug