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> CD73 Protein, Human, Recombinant (His & Avi), Biotinylated V2

CD73 Protein, Human, Recombinant (His & Avi), Biotinylated V2

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Cat# NB-64-128469-50ug

Size : 50ug

Brand : Neo Biotech

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CD73 Protein, Human, Recombinant (His & Avi), Biotinylated V2

(Synonyms: NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)) Copy Product Info

Synonyms: NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)

Catalog No. TMPY-06907U Copy Product Info
CD73 Protein, Human, Recombinant (His & Avi), Biotinylated V2 is expressed in HEK293 Cells. The accession number is P21589-1.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Immobilized Biotinylated Human CD73, His Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 2.5 ng/ml determined by ELISA.
Description
CD73 Protein, Human, Recombinant (His & Avi), Biotinylated V2 is expressed in HEK293 Cells. The accession number is P21589-1.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP21589-1
ConstructionTrp27-Lys547
Protein Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsNTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)
Research Background
CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Chemical Properties
Molecular Weight60.92 kDa (Predicted); 60-70 kDa (Due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

TMPY-06907NT-5NT 5CD-73CD 735'-nucleotidase, ecto
Related Tags: CD73 Protein, Human, Recombinant (His & Avi), Biotinylated V2 chemical structure | CD73 Protein, Human, Recombinant (His & Avi), Biotinylated V2 molecular weight