CDH6 ELISA Kit (Human)

Cat# OKEH01820

Size : 96T

Brand : Aviva Systems Biology

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CDH6 ELISA Kit (Human) (OKEH01820)

Datasheets/ManualsPrintable datasheet for CDH6 ELISA Kit (Human) (OKEH01820)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CDH6 ELISA Kit (Human) (OKEH01820) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDH6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDH6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDH6 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
CDH6 Microplate96 Wells (12 x 8 Well strips)
CDH6 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CDH6 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Cadherin-6
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCDH6
Gene Full Namecadherin 6
Alias SymbolsCAD6, cadherin 6, type 2, K-cadherin (fetal kidney), cadherin-6, KCAD, Kidney cadherin.
NCBI Gene Id1004
Protein NameCadherin-6
Description of TargetCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Uniprot IDP55285
Protein Accession #NP_004923.1
Nucleotide Accession #NM_004932.3
Protein Size (# AA)790
Molecular Weight88 kDa

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