fr en

Reagents and instruments for immunology, cell biology and molecular biology.

Ceftobiprole [209467-52-7]

Print

Cat# HY-112579-50mg

Size : 50mg

Contact local distributor :

Phone : +1 850 650 7790

Brand : MedChemExpress

Request more information

Contact local distributor :

Phone : +1 850 650 7790

Description

Ceftobiprole (Ro 63-9141) is a broad-spectrum cephalosporin with high levels of in vitro activity against methicillin- (MRSA) and vancomycin-resistant staphylococci (VRSA) and penicillin-resistant streptococci with a MIC₉₀ value of 2 μg/mL for MRSA. Ceftobiprole also inhibits gram-positive and gram-negative pathogens. Ceftobiprole can be used for the study of hospital-acquired pneumonia (excluding ventilator-associated pneumonia) and community-acquired pneumonia^[1]^[2]^[3].

IC₅₀ & Target

β-lactam

Cellular Effect

Cell Line	Type	Value	Description	References
THP-1	EC₅₀	0.07 mg/L Compound: BPR	Antimicrobial activity against methicillin-susceptible Staphylococcus aureus ATCC 25293 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against methicillin-susceptible Staphylococcus aureus ATCC 25293 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	0.07 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus N4042228 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus N4042228 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	0.08 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus N4090440 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus N4090440 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	0.19 mg/L Compound: BPR	Antimicrobial activity against methicillin-susceptible Staphylococcus aureus NRS52 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against methicillin-susceptible Staphylococcus aureus NRS52 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	0.2 mg/L Compound: BPR	Antimicrobial activity against methicillin-susceptible Staphylococcus aureus ATCC 25293 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against methicillin-susceptible Staphylococcus aureus ATCC 25293 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.2 mg/L Compound: BPR	Antimicrobial activity against methicillin-susceptible Staphylococcus aureus ATCC 11632 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against methicillin-susceptible Staphylococcus aureus ATCC 11632 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.2 mg/L Compound: BPR	Antimicrobial activity against methicillin-susceptible Staphylococcus aureus NRS52 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against methicillin-susceptible Staphylococcus aureus NRS52 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.205 mg/L Compound: BPR	Antimicrobial activity against methicillin-susceptible Staphylococcus aureus ATCC 11632 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against methicillin-susceptible Staphylococcus aureus ATCC 11632 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	0.23 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired vancomycin-intermediate Staphylococcus aureus NRS126 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against hospital-acquired vancomycin-intermediate Staphylococcus aureus NRS126 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	0.25 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired vancomycin-resistant Staphylococcus aureus VRS1 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against hospital-acquired vancomycin-resistant Staphylococcus aureus VRS1 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	0.26 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired vancomycin-intermediate Staphylococcus aureus NRS18 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against hospital-acquired vancomycin-intermediate Staphylococcus aureus NRS18 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	0.28 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 33591 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 33591 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	0.28 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 33591 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 33591 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.28 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 33592 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 33592 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.28 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 433000 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 433000 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.28 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired vancomycin-intermediate Staphylococcus aureus NRS18 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against hospital-acquired vancomycin-intermediate Staphylococcus aureus NRS18 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.28 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired vancomycin-intermediate Staphylococcus aureus NRS126 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against hospital-acquired vancomycin-intermediate Staphylococcus aureus NRS126 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.28 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired vancomycin-resistant Staphylococcus aureus VRS1 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against hospital-acquired vancomycin-resistant Staphylococcus aureus VRS1 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.33 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus NRS192 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus NRS192 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.33 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus N4090440 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus N4090440 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.33 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus N4042228 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus N4042228 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.33 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus STA 44 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus STA 44 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.33 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus STA 228 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus STA 228 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.33 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus isolate MEH2225605 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4 Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus isolate MEH2225605 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 7.4	[PMID: 19289525]
THP-1	EC₅₀	0.41 mg/L Compound: BPR	Antimicrobial activity against methicillin-susceptible Staphylococcus aureus ATCC 25293 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against methicillin-susceptible Staphylococcus aureus ATCC 25293 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.41 mg/L Compound: BPR	Antimicrobial activity against methicillin-susceptible Staphylococcus aureus ATCC 11632 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against methicillin-susceptible Staphylococcus aureus ATCC 11632 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.41 mg/L Compound: BPR	Antimicrobial activity against methicillin-susceptible Staphylococcus aureus NRS52 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against methicillin-susceptible Staphylococcus aureus NRS52 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.54 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus NRS192 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus NRS192 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.54 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus N4090440 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus N4090440 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.54 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus N4042228 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus N4042228 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.54 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus STA 44 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus STA 44 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.54 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus STA 228 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus STA 228 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.54 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus isolate MEH2225605 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus isolate MEH2225605 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.7 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus NRS192 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus NRS192 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	0.73 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 433000 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 433000 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	0.77 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired vancomycin-intermediate Staphylococcus aureus NRS126 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against hospital-acquired vancomycin-intermediate Staphylococcus aureus NRS126 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.77 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired vancomycin-resistant Staphylococcus aureus VRS1 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against hospital-acquired vancomycin-resistant Staphylococcus aureus VRS1 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.77 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 33591 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 33591 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.77 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 33592 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 33592 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.77 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 433000 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 433000 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.77 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired vancomycin-intermediate Staphylococcus aureus NRS18 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5 Antimicrobial activity against hospital-acquired vancomycin-intermediate Staphylococcus aureus NRS18 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs at pH 5.5	[PMID: 19289525]
THP-1	EC₅₀	0.82 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus isolate MEH2225605 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus isolate MEH2225605 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	0.88 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus STA 228 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus STA 228 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	0.94 mg/L Compound: BPR	Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus STA 44 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against Panton-valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus STA 44 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]
THP-1	EC₅₀	1.23 mg/L Compound: BPR	Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 33592 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs Antimicrobial activity against hospital-acquired methicillin-resistant Staphylococcus aureus ATCC 33592 infected in human THP-1 cells assessed as change in bacterial count after 24 hrs	[PMID: 19289525]

In Vitro

Ceftobiprole (Ro 63-9141) has demonstrates activity against important gram-positive bacteria, including S. pneumonia (PRSP), Methicillin-resistant S. aureus (MRSA), and E. faecalis with MIC₉₀ values of 0.25, 2, and 2 mcg/mL, respectively. Ceftobiprole has also demonstrated potent in vitro activity against several clinical isolates of community-associated Methicillin-resistant S. aureus (CA-MRSA), S. aureus (VISA), and S. aureus (VRSA), with a minimum inhibitory concentration (MIC) of 2 mcg/mL^[1].
Ceftobiprole is highly active against S. aureus, withMICs ranging from 0.12 to 4 mg/L (only one resistant strain,MIC of 4 mg/L). Furthermore, Ceftobiprole is twice more active on Methicillin-susceptible S. aureus (MSSA) strains with MIC₅₀ and MIC₉₀ of 0.5 mg/L than on MRSA strains with MIC₅₀ and MIC₉₀ of 1 mg/L. Moreover, Panton-Valentine leukocidin (PVL)+MRSA are slightly more susceptible to Ceftobiprole (MIC₅₀ of 0.5 mg/L and MIC₉₀ of 1 mg/L) than PVL-MRSA (MIC₅₀ and MIC₉₀ of 1 mg/L)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solvent & Solubility

In Vitro:

DMSO : 4.95 mg/mL (9.26 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8707 mL	9.3533 mL	18.7066 mL
5 mM	0.3741 mL	1.8707 mL	3.7413 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.5 mg/mL (0.94 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.5 mg/mL (0.94 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.5 mg/mL (0.94 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Purity & Documentation

Purity: 97.22%

Data Sheet (277 KB) SDS (393 KB)

COA (253 KB) HNMR (279 KB) RP-HPLC (207 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Kisgen J, et al. Ceftobiprole, a Broad-Spectrum Cephalosporin With Activity against Methicillin-Resistant Staphylococcus aureus (MRSA). P T. 2008 Nov;33(11):631-41. [Content Brief]

[2]. Hodille E, et al. In vitro activity of ceftobiprole on 440 Staphylococcus aureus strains isolated from bronchopulmonary infections. Med Mal Infect. 2017 Mar;47(2):152-157. [Content Brief]

[3]. E Azoulay-Dupuis, et al. Efficacy of BAL5788, a prodrug of cephalosporin BAL9141, in a mouse model of acute pneumococcal pneumonia. Antimicrob Agents Chemother. 2004 Apr;48(4):1105-11. [Content Brief]

You might also be interested by the following products:

Cat#

Description

Cond.

Price Bef. VAT

HY-66011-50mg

Moxifloxacin (Hydrochloride) [186826-86-8]

50mg

Ceftobiprole [209467-52-7]

Cat# HY-112579-50mg

Contact local distributor :

Brand : MedChemExpress

Contact local distributor :

Ceftobiprole Related Antibodies

You might also be interested by the following products: