RayBio® COVID-19 (SARS-CoV-2) Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgG antibody against the Delta variant of SARS-CoV-2 in serum.
COVID-19 Delta Spike Variant Human IgG ELISA Kit
COVID-19 Delta Spike Variant Human IgG ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Specificity | This indirect ELISA kit shows cross-reactivity with other SARS-CoV-2 Spike variants. |
| Compatible Sample Types | Serum |
| Gene Symbols | IGG |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Indirect |
| Method Of Detection | Colorimetric |
| Research Area | Infectious Disease, COVID-19 |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | -80°C |
Application Notes
Kit Components
- SARS-CoV-2 Delta spike variant protein coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Anti-Human IgG
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 μl prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
- Add 100 μl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.
