Dimethylsulfoxide (DMSO) for Cell Culture

Cat# D8050-01-100ml

Size : 100ml

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


D8050-01 Dimethylsulfoxide (DMSO) for Cell Culture

Grade
Cell Culture Grade
Shipping Temp
RT
Storage Temp
RT

Ready-to-use sterile filtered product conveniently packaged in septum screw-capped amber bottles.

DMSO has been used as a cryoprotectant and is still an important constituent of cryoprotectant vitrification mixtures used to preserve organs, tissues, and cell suspensions. Without it, up to 90% of frozen cells will become inactive. It is particularly important in the freezing and long-term storage of embryonic stem cells and hematopoietic stem cells, which are often frozen in a mixture of 10% DMSO, a freezing medium, and 30% fetal bovine serum. In the cryogenic freezing of heteroploid cell lines (MDCK, VERO, etc.) a mixture of 10% DMSO with 90% EMEM (70% EMEM + 30% fetal bovine serum + antibiotic mixture) is used.

Synonyms:
Methyl Sulfoxide; Dimethylsulfoxide; DMSO

CAS No:
67-68-5

Molecular Formula:
C2H6SO

Molecular Weight:
78.13

Purity (GC):
≥99%

Specific Gravity:
1.095-1.097

Congealing Temp:
18.3°C

Refractive Index:
1.4755-1.4780

Water:
≤0.1%

Titratable Acid:
≤5ml

Residue after Evaporation:
≤0.01%

Absorbance:
To Pass ACS Specifications

Substances Darkened by KOH:
To Pass ACS Specifications

RNase:
None Detected

DNase:
None Detected

Endotoxin:
None Detected

Note: DMSO will congeal easily and may remain a solid at cool temperatures. Warm slightly to return to liquid state.

Applications
Important Note: |This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. ||Toxicity and Hazards: |All products should be handled by qualified personnel only, trained in laboratory procedures.
References
Pegg, DE. (2007). Day JG, Stacey GN, ed. "Principles of Cryopreservation". Cryopreservation and Freeze-Drying Protocols, Second Edition. Methods in Molecular Biology (Humana Press) 368: 39–57. doi:10.1007/978-1-59745-362-2_3. ISBN 978-1-58829-377-0. ISSN 1064-3745. PMID 18080461.

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