Size : 1500units
Phone : +1 850 650 7790
Deoxyribonuclease I (DNase I) is an endonuclease that digests single- and double-stranded DNA and chromatin (reaction rate is restricted by DNA association with histones). It functions by hydrolyzing phosphodiester bonds, producing mono and oligodeoxyribonucleotides with a 5'-phosphate and a 3'-hydroxyl group. Its activity depends on Ca2+ and it is activated by Mg2+ or Mn2+. DNase I with Mg2+ cleaves randomly double-stranded DNA at any site. DNase I with Mn2+ cleaves two DNA strands at approximately the same site to form sticky ends with 1-2 nucleotide overhangs or form blunt ends.
Concentration: 3 units/μl
Storage
at -18℃ or below for two years
Shipping
Dry ice (-70℃).
1 Ma L, Yang Y, Wang Y, et al. SlRBP1 promotes translational efficiency via SleIF4A2 to maintain chloroplast function in tomato[J]. The Plant Cell, 2022.(IF 11.27)
2 Li Z, Huang P, Wang M, et al. Stepwise increase of thaxtomins production in Streptomyces albidoflavus J1074 through combinatorial metabolic engineering[J]. Metabolic Engineering, 2021.(IF 9.78)
Manual