Endogenous Peroxidase Blocking Buffer
Cat# MBS2567132-50mL
Size : 50mL
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
Endogenous Peroxidase IHC Related Reagent
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2567132MBS2567132 is an IHC related reagent targeting Endogenous Peroxidase. Also known as Endogenous Peroxidase Blocking Buffer. More details
Product Overview
Product Name
Endogenous Peroxidase, IHC Related Reagent
Full Product Name
Endogenous Peroxidase Blocking Buffer
Preparation and Storage
Store at 2-8 degree C. Stable for ~12 months. Avoid of freezing.
The reagents are valid within 6 months after opening.
The reagents are valid within 6 months after opening.
Introduction
Endogenous Peroxidase Blocking Buffer is mainly used to block endogenous peroxidase in tissues or cells during immunohistochemistry (IHC), immunocytochemistry (ICC) and in situ hybridization (ISH). Endogenous peroxidase is widely found in some cells and tissues, including red blood cells, kidney and liver, which will result with a high background and even false positive results when using peroxidase method to detect samples. Therefore, cell or tissue samples should be blocked with appropriate peroxidase blocking solution before staining to eliminate the interference of endogenous peroxidase. The product is 3% H2O2, which is a ready-to-use buffer to block the endogenous hydrogen peroxide of the sample without dilution.
Experimental Procedure
1. For IHC or ICC
1) Paraffin slices need to be dewaxed, hydrated and then repaired. Cell slices and frozen slices can neglect this step.
2) Suck up the liquid on the surface of the slice, drop appropriate amount of Endogenous Peroxidase Blocking Buffer to cover the sample completely, and incubate at RT for 5~10 min or 37°C for 2~5 min.
3) Remove the Endogenous Peroxidase Blocking Buffer, wash the sample with washing solution or PBS and other appropriate solutions for 1~3 min/time, 2~3 times. Then, follow the steps, such as blocking and primary antibody incubation.
2. For ISH
1) Drop appropriate amount of Endogenous Peroxidase Blocking Buffer to cover the sample completely, and incubate at RT for 5~10 min or 37°C for 2~5 min.
2) Remove the blocking buffer, wash with the washing solution of in situ hybridization for 2~5 min/time, 2~3 times. Then follow the steps such as hybridization.
1) Paraffin slices need to be dewaxed, hydrated and then repaired. Cell slices and frozen slices can neglect this step.
2) Suck up the liquid on the surface of the slice, drop appropriate amount of Endogenous Peroxidase Blocking Buffer to cover the sample completely, and incubate at RT for 5~10 min or 37°C for 2~5 min.
3) Remove the Endogenous Peroxidase Blocking Buffer, wash the sample with washing solution or PBS and other appropriate solutions for 1~3 min/time, 2~3 times. Then, follow the steps, such as blocking and primary antibody incubation.
2. For ISH
1) Drop appropriate amount of Endogenous Peroxidase Blocking Buffer to cover the sample completely, and incubate at RT for 5~10 min or 37°C for 2~5 min.
2) Remove the blocking buffer, wash with the washing solution of in situ hybridization for 2~5 min/time, 2~3 times. Then follow the steps such as hybridization.
Cautions
1. For some special cases where it is difficult to use the peroxidase detection system, please try to use the alkaline phosphatase detection system.
2. This product couldn't be used for clinical diagnosis or treatment, food or medicine, and can't be stored in residence.
3. For your safety and health, please wear the lab coat and disposable gloves before the experiments
2. This product couldn't be used for clinical diagnosis or treatment, food or medicine, and can't be stored in residence.
3. For your safety and health, please wear the lab coat and disposable gloves before the experiments
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for Endogenous Peroxidase ihc related reagent
Immunohistochemistry (IHC), Immunocitochemistry (ICC), In Situ Hybridization (ISH)
Other Notes
Small volumes of Endogenous Peroxidase ihc related reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.