Glucose-6-Phosphate (G6P) is glucose sugar phosphorylated on carbon 6. Most of the glucose entering cells is phosphorylated to G6P. G6P has three primary fates within the cell. It lies at the start of two major metabolic pathways: glycolysis and the pentose phosphate pathway. In addition to these metabolic pathways, glucose 6-phosphate may also be converted to glycogen or starch for storage. The Glucose-6-Phosphate Assay Kit provides a simple, and automation-ready procedure for measuring G6P concentration. G6P is oxidized by glucose-
6-phosphate dehydrogenase and the formed NADPH is coupled to the formazan (WST-8) chromogen. The intensity of the product color, measured at 460nm, is proportional to the G6P concentration in the sample.

Intended Use:
For quantitative colorimetric determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism.

Applications:
G6P determination in biological samples (e.g. plasma, serum, tissue and culture media.)

Range:
10-1000um G6P

Detection limit:
10um

Kit Components:
Assay Buffer, 1x10ml
NADP/WST8, 1x1ml
Standard (100mM G6P), 1x100ul
Enzyme A, 1x120ul
Enzyme B, 1x120ul

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.