RayBio® Human Adiponectin (ACRP30) ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Human Adiponectin ELISA Kit
Human Adiponectin ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 25 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 25pg/ml - 18,000pg/ml |
| Specificity | Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 30,000 fold |
| Accession Number | Q15848 |
| Gene Id | 9370 |
| Gene Symbols | ADIPOQ, ACRP30, ACDC, APM1, GBP28 |
| Protein Name / Synonyms | Adiponectin (30 kDa adipocyte complement-related protein) (Adipocyte complement-related 30 kDa protein) (ACRP30) (Adipocyte, C1q and collagen domain-containing protein) (Adipose most abundant gene transcript 1 protein) (apM-1) (Gelatin-binding protein) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Target Background
Adiponectin is a 244-amino acid protein secreted mainly by adipose tissue, but expression is also seen in osteoblasts, liver parenchyma cells, myocytes, epithelial cells, and placental tissue. The biological actions of adiponectin are directed through the receptors AdipoR1, AdipoR2 and T-cadherin. Serum levels of adiponectin decrease with obesity and are positively linked with insulin sensitivity. Low levels of this anti-inflammatory adipokine can produce a chronic state of low-grade inflammation which can lead to insulin resistance, diabetes, hypertension, and atherosclerosis.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
citations
Blenderized watermelon consumption decreases body mass index, body mass index percentile, body fat and HbA1c in children with overweight or obesity
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Pediatric Obesity
Obesity as a modifying factor of periodontal therapy outcomes: local and systemic adipocytokines and oxidative stress markers
- Species:
- Human
- Sample Type:
- gingival crevicular fluid (GCF), Serum
- Year:
- 2023
- Journal Name:
- Clinical Oral Investigations
Association of serum adiponectin levels and ADIPOQ SNP rs2241766 with breast cancer risk in Indian women
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of Cancer Research and Therapeutics
Effects of mixed nut consumption on LDL cholesterol, lipoprotein(a), and other cardiometabolic risk factors in overweight and obese adults
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Nutrition Metabolism and Cardiovascular Diseases
Periodontal status and whole salivary adipokines after scaling and root planing with and without adjunct antimicrobial photodynamic therapy in obese patients with periodontitis
- Species:
- Human
- Sample Type:
- whole salivary
- Year:
- 2022
- Journal Name:
- Photodiagnosis and Photodynamic Therapy
Risk analysis of systemic levels of estrogen and adipokines as well as estrogen receptors from PBMCs in childbearing and perimenopausal women with obesity
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Diabetes Metabolic Syndrome and Obesity-Targets and Therapy
Mirna 326 Derived Exosomes Is A Potential Modulator Of Adiponectin In Diabetes Mellitus
- Species:
- Human
- Sample Type:
- Plasma (Exosomes)
- Year:
- 2020
- Journal Name:
- European Journal of Molecular and Clinical Medicine
Lima, Cyro Brito, Laisa Ribeiro, Fernanda Agapito SimÃμes, Guaraci Requena, Liania Alves Luzia, Patricia Helen Rondó, Cord blood concentrations of leptin, zinc-2-glycoprotein and adiponectin, and adiposity gain in the first three months of life, Nutrition (2018), https://doi.org/10.1016/j.nut.2018.02.012.
- Species:
- Human
- Sample Type:
- Plasma (Cord blood)
- Year:
- 2018
Concentrations of leptin, adiponectin and other metabolic parameters in non-obese children with Down syndrome.
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2017
- Journal Name:
- Journal of Pediatric Endocrinology and Metabolism
“Lack of adiponectin and adiponectin receptor 1 contributes to benign prostatic hyperplasia.†Oncotarget vol
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2017