RayBio® Human IL-2 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
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Human IL-2 ELISA Kit
Human IL-2 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 4 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 4 pg/ml - 1500 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P60568 |
| Gene Id | 3558 |
| Gene Symbols | IL2 |
| Protein Name / Synonyms | Interleukin-2 (IL-2) (T-cell growth factor) (TCGF) (Aldesleukin) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation, Antibody Drug Development, Protein Therapeutics |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
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Target Background
IL-2 is a 15 kDa glycoprotein that is produced by helper T cells to promote immunostimulatory or immunoinhibitory activity depending on target cells. It can activate the cytotoxic function of natural killer cells, T lymphocytes and monocytes to enhance the cytotoxicity of these effector cells. Once secreted IL-2 will bind to neighboring cells that harbor its receptor, IL-2R. The IL-2 receptors couples to JAK tyrosine kinases and activates the STAT5 transcription factors. IL-2 therapy has shown to be effective in patients with autoimmune diseases.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
The application of HER2 and CD47 CAR-macrophage in ovarian cancer
- Species:
- Human
- Sample Type:
- THP-1 cell
- Year:
- 2023
- Journal Name:
- Journal of Translational Medicine
Clinico-Epidemiological Laboratory Findings of COVID- 19 Positive Patients in a Hospital in Saudi Arabia
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Infection and drug resistance
Dendritic cell-mimicking scaffolds for ex vivo T cell expansion
- Species:
- Human
- Sample Type:
- T cell
- Year:
- 2022
- Journal Name:
- Bioactive materials
RNA N6-Methyladenosine (m6A) Methyltransferase-like 3 Facilitates Tumorigenesis and Cisplatin Resistance of Arecoline-Exposed Oral Carcinoma
- Species:
- Human
- Sample Type:
- oral squamous cell carcinoma (OSCC) tissue
- Year:
- 2022
- Journal Name:
- Cells
Bispecific T-cell engager (BiTE) immunotherapy of ovarian cancer based on MIL-88A MOF/MC gene delivery system
- Species:
- Human
- Sample Type:
- Conditioned Media (T cells stimulatied with anti-CD3 and anti-Epcam BITE)
- Year:
- 2020
IL-6, IL-18, sIL-2R, and TNF proinflammatory markers in depression and schizophrenia patients who are free of overt inflammation
- Species:
- Human
- Sample Type:
- Serum (Depressed Patients)
- Year:
- 2015
- PubMed ID:
- 25985379
Immunological Study of Women Infected with Trichomonas vaginalis Parasite in Baghdad city
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2014
The protein pheromone Er-1 of the ciliate Euplotes raikovi stimulates human T-cell activity: involvement of interleukin-2 system.
- Species:
- Human
- Sample Type:
- Conditioned Media (jurkat cells)
- Year:
- 2013
- Journal Name:
- Experimental Cell Research
The Role of Cytokines among Women with Spontaneous Miscarriage.
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2013
- Journal Name:
- Medical Journal of Islamic World Academy of Sciences
Upregulated TCR Enhances Interleukin-2 Production in T-Cells from Patients with CML
- Species:
- Human
- Sample Type:
- Conditioned Media (PBMCs with a plasmid IRES Site)
- Year:
- 2012
- Journal Name:
- Dna and Cell Biology
- PubMed ID:
- 23057733