RayBio® Human IP-10 (CXCL10) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human IP-10 ELISA Kit
Human IP-10 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 2.5 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 8.23 pg/ml - 6000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP- 2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P02778 |
| Gene Id | 3627 |
| Gene Symbols | CXCL10, INP10, SCYB10 |
| Protein Name / Synonyms | C-X-C motif chemokine 10 (10 kDa interferon gamma-induced protein) (Gamma-IP10) (IP-10) (Small-inducible cytokine B10) [Cleaved into: CXCL10(1-73)] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
citations
Brexanolone therapeutics in post-partum depression involves inhibition of systemic inflammatory pathways
- Species:
- Human
- Sample Type:
- blood cell
- Year:
- 2023
- Journal Name:
- EBioMedicine
Radiotherapy enhances CXCR3highCD8+ T cell activation through inducing IFNγ-mediated CXCL10 and ICAM-1 expression in lung cancer cells
- Species:
- Human
- Sample Type:
- Peripheral blood mononuclear cells, A549 cell
- Year:
- 2023
- Journal Name:
- Cancer Immunology Immunotherapy
Human induced pluripotent stem cell engineering establishes a humanized mouse platform for pediatric low-grade glioma modeling
- Species:
- Human
- Sample Type:
- human induced pluripotent stem cell (hiPSC)
- Year:
- 2022
- Journal Name:
- Acta neuropathologica communications
Dysfunction of brain pericytes in chronic neuroinflammation
- Species:
- Human
- Sample Type:
- Conditioned Media (HIV innoculated brain periocytes)
- Year:
- 2016
- Journal Name:
- Journal of Cerebral Blood Flow and Metabolism
- PubMed ID:
- 26661157
Novel inflammatory markers associated with cognitive performance: Singapore Longitudinal Ageing Studies
- Species:
- Human
- Sample Type:
- Plasma (Cognitive Performance)
- Year:
- 2016
- Journal Name:
- Neurobiology of Aging
- PubMed ID:
- 26923410
CXCL10/IP-10 is a Biomarker and Mediator for Kawasaki Disease
- Species:
- Human
- Sample Type:
- Plasma (Kawasaki's Disease patients)
- Year:
- 2015
- Journal Name:
- Circulation Research
- PubMed ID:
- 25605650
STAT3 inhibition reduces toxicity of oncolytic VSV and provides a potentially synergistic combination therapy for hepatocellular carcinoma
- Species:
- Human
- Sample Type:
- Conditioned Media (Carcinoma Cell lines treated with a Stat3 inhibitor or control)
- Year:
- 2015
- Journal Name:
- Cancer Gene Therapy
- PubMed ID:
- 25930184
Peripheral Blood Cells from Patients with Autoimmune Addison's Disease Poorly Respond to Interferons In Vitro, Despite Elevated Serum Levels of Interferon-Inducible Chemokines
- Species:
- Human
- Sample Type:
- Plasma (Adddison disease that poorly respond to IFNs); Serum (Adddison disease that poorly respond to IFNs)
- Year:
- 2015
- Journal Name:
- Journal of Interferon and Cytokine Research
- PubMed ID:
- 25978633
Diagnostic Value of Circulating CXC Chemokines in Non-small Cell Lung Cancer
- Species:
- Human
- Sample Type:
- Serum (Non small cell lung cancer patients vs controls)
- Year:
- 2015
- Journal Name:
- Anticancer Research
- PubMed ID:
- 26637925
Diagnosing latent tuberculosis in immunocompromised patients measuring blood IP-10 production capacity: an analysis of chronic renal failure patients
- Species:
- Human
- Sample Type:
- Plasma (Quantiferon-TB Gold in tube test kit blood samples)
- Year:
- 2015
- Journal Name:
- Internal Medicine
- PubMed ID:
- 25758071
