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> Human MMP-1 ELISA Kit

Human MMP-1 ELISA Kit

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Cat# ELH-MMP1-1

Size : 1PlateKit

Brand : RayBiotech

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Phone : +1 850 650 7790

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Human MMP-1 ELISA Kit Standard CurveHuman MMP-1 ELISA Kit Linearity ResultsHuman MMP-1 ELISA Kit Spiking and Recovery ResultsHuman MMP-1 ELISA KitELH-MMP1 was used to measure MMP1 secretion in human gingival fibroblasts treated with cholesterol. Figure adapted from Tran et al., Experimental & Molecular Medicine, 2023.ELH-MMP1 was used to measure MMP-1 levels in human gingival fibroblast culture media following lipopolysaccharide stimulation with or without S-1-propenyl-L-cysteine treatment. Figure adapted from Nango et al., PLOS ONE, 2023.ELH-MMP1 was used to measure MMP-1 levels in culture supernatants from human hip chondrocytes (osteoarthritis vs non-osteoarthritis donors) under IL-1β or TNF stimulation. Figure adapted from Eitner et al., Biomedicines, 2022.ELH-MMP1 was used to measure MMP-1 levels in fibroblast-like synoviocyte culture supernatants following Hedgehog pathway siRNA treatment. Figure adapted from Lin et al., Molecular Therapy – Nucleic Acids, 2023.ELH-MMP1 was used to measure MMP1 levels in MG63 osteoblast-like cell culture supernatants following cold atmospheric plasma treatment. Figure adapted from Eggers et al., Odontology, 2020.Human MMP-1 ELISA KitHuman MMP-1 ELISA KitHuman MMP-1 ELISA Kit5 Plate Kit Packaging (one box)

Human MMP-1 ELISA Kit Standard Curve

Human MMP-1 ELISA Kit

RayBio® Human MMP-1 ELISA Kit for cell culture supernatants, heparin treated plasma, serum, and lysate samples. EDTA and Citrate are not recommended.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
8 pg/ml

Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
8 pg/ml - 18000 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-2, - 3, -9, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Recommended Dilution (Serum/Plasma)
2 - 10 fold
Accession Number
P03956
Gene Id
4312
Gene Symbols
MMP1, CLG
Protein Name / Synonyms
Interstitial collagenase (EC 3.4.24.7) (Fibroblast collagenase) (Matrix metalloproteinase-1) (MMP-1) [Cleaved into: 22 kDa interstitial collagenase 27 kDa interstitial collagenase]
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Shipping TypeBlue ice
Storage≤-20°C

Target Background

MMP-1 is a member of the large family of matrix metalloproteinase (MMP) that degrades substrates, such as gelatin, nidogen, collagen type 3, collagen type 4, laminin-1, proteoglycans, elastin, etc. Most MMP proteins are activated after being cleaved by extracellular proteinases. The matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological pathway: embryonic development, reproduction, tissue remodeling. MMP is also involved in disease processes, such as arthritis and metastasis.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

ELH-MMP1 was used to measure MMP1 secretion in human gingival fibroblasts treated with cholesterol. Figure adapted from Tran et al., Experimental & Molecular Medicine, 2023.

ELH-MMP1-1 was used to measure MMP-1 levels in human gingival fibroblast culture media following lipopolysaccharide stimulation with or without S-1-propenyl-L-cysteine treatment. Figure adapted from Nango et al., PLOS ONE, 2023.

ELH-MMP1 was used to measure MMP-1 levels in culture supernatants from human hip chondrocytes (osteoarthritis vs non-osteoarthritis donors) under IL-1β or TNF stimulation. Figure adapted from Eitner et al., Biomedicines, 2022.

ELH-MMP1-1 was used to measure MMP-1 levels in fibroblast-like synoviocyte culture supernatants following Hedgehog pathway siRNA treatment. Figure adapted from Lin et al., Molecular Therapy – Nucleic Acids, 2023.

ELH-MMP1-1 was used to measure MMP1 levels in MG63 osteoblast-like cell culture supernatants following cold atmospheric plasma treatment. Figure adapted from Eggers et al., Odontology, 2020.

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate

Additional Materials Required

  • Distilled or deionized water
  • Precision pipettes to deliver 2 µL to 1 µL volumes
  • Adjustable 1-25 µL pipettes for reagent preparation
  • 100 µL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µL of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

citations

  • Disruption of cholesterol homeostasis triggers periodontal inflammation and alveolar bone loss

    Year:
    2023
    Journal Name:
    Experimental and Molecular Medicine
    PubMed ID:
    38036731

  • Eosinophil-derived galectin-10 upregulates matrix metalloproteinase expression in bullous pemphigoid blisters

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Journal of Dermatological Science

  • S-1-propenyl-L-cysteine suppresses lipopolysaccharide-induced expression of matrix metalloproteinase-1 through inhibition of tumor necrosis factor-α converting enzyme-epidermal growth factor receptor axis in human gingival fibroblasts

    Species:
    Human
    Sample Type:
    HGF-1 cell
    Year:
    2023
    Journal Name:
    Plos One

  • Biological Activities of Citrus aurantium Leaf Extract by Optimized Ultrasound-Assisted Extraction

    Species:
    Human
    Sample Type:
    CCD-966SK cell
    Year:
    2023
    Journal Name:
    Molecules

  • Contrast-Enhanced Computed Tomography and Laboratory Parameters as Non-Invasive Diagnostic Markers of Pancreatic Fibrosis

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Diagnostics

  • Ex Vivo Analysis of an Association of Mechanical Strength of Dilated Ascending Aorta with Tissue Matrix Metalloproteinases and Cytokines

    Species:
    Human
    Sample Type:
    aortic
    Year:
    2023
    Journal Name:
    BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE

  • The FoxP1 gene regulates lung function, production of matrix metalloproteinases and inflammatory mediators, and viability of lung epithelia

    Species:
    Human
    Sample Type:
    BEAS-2B cell
    Year:
    2022
    Journal Name:
    Respiratory Research

  • Osteoarthritis-Induced Metabolic Alterations of Human Hip Chondrocytes

    Year:
    2022
    Journal Name:
    Biomedicines
    PubMed ID:
    35740371

  • Mécano-Stimulation™ of the skin improves sagging score and induces beneficial functional modification of the fibroblasts: clinical, biological, and histological evaluations

    Species:
    Human
    Sample Type:
    fibroblasts
    Year:
    2022
    Journal Name:
    Clinical interventions in aging

  • Effect of Electro-Acupuncture (EA) and Manual Acupuncture (MA) on Markers of Inflammation in Knee Osteoarthritis

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2022
    Journal Name:
    Journal of pain research

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