Reagents and instruments for immunology, cell biology and molecular biology.
 
> Human TGF-beta 1 ELISA Kit

Human TGF-beta 1 ELISA Kit

Print Print
Please log in

Cat# ELH-TGFb1-1

Size : 1PlateKit

Brand : RayBiotech

Contact local distributor :


Phone : +1 850 650 7790

Best Seller
Human TGF-beta 1 ELISA Kit Standard CurveHuman TGF-beta 1 ELISA KitHuman TGF-beta 1 ELISA KitELH-TGFb1 was used to measure TGF-β levels in apical and basolateral secretions from mock- and measles virus–infected human airway epithelial cultures at 3 days post-infection. Figure adapted from Hippee et al., J Virol, 2025.ELH-TGFb1 was used to measure TGF-β1 levels in conditioned media from stromal fibroblasts and prostate cancer cell co-culture experiments. Figure adapted from Pu et al., Sci Adv., 2024.ELH-TGFb1 was used to measure TGF-β1 levels in conditioned media from oral squamous cell carcinoma cells following EZH2 inhibition. Figure adapted from Campolo et al., Pharmaceuticals (Basel), 2024.ELH-TGFb1 was used to measure TGF-β in conditioned media from CD4+ T lymphocytes co-cultured with cytokine-treated human periodontal ligament-derived mesenchymal stromal cells. Figure adapted from Behm et al., Frontiers in Immunology, 2023.ELH-TGFb1 was used to measure TGF-β1 in culture media from RalGAPβ-deficient and control pancreatic ductal adenocarcinoma cell lines. Figure adapted from Cao et al., Journal of Biological Chemistry, 2023.ELH-TGFb1 and ELH-TGFb2 were used to measure TGF-β1 and TGF-β2 in cervicovaginal lavage samples from women with infertility or habitual abortion. Table adapted from Fernández et al., Nutrients, 2023.ELH-TGFb1 was used to measure TGFβ in conditioned media from breast cancer stem cells and non-stem cancer cells. Figure adapted from Mukherjee et al., Discover Oncology, 2023.Human TGF-beta 1 ELISA KitHuman TGF-beta 1 ELISA KitHuman TGF-beta 1 ELISA Kit5 Plate Kit Packaging (one box)

Human TGF-beta 1 ELISA Kit Standard Curve

Human TGF-beta 1 ELISA Kit

TGF beta 1 ELISA Kit for cell culture supernatants, plasma, and serum samples. Pre-coated 96-well strip microplate kit. 18 pg/ml sensitivity.
Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit, 10 Plate Kit
SpeciesHuman
Sensitivity
18 pg/ml

Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
18 pg/ml - 4000 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1alpha, TIMP-1, TIMP-2, TNF-alha, TGF-beta 2, TGF-beta 3, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
20 fold after treatment
Accession Number
P01137
Gene Id
7040
Gene Symbols
TGFB1, TGFB
Protein Name / Synonyms
Transforming growth factor beta-1 (TGF-beta-1) [Cleaved into: Latency-associated peptide (LAP)]
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaAngiogenesis, Cardiovascular Disease, Cancer, Inflammation, TGF-beta Signaling
Shipping TypeBlue ice
Storage≤-20°C

Target Background

Transforming growth factor β1 (TGFB1) is a cytokine that has an important role in a number of different cellular functions, such as, proliferation, differentiation, chemotaxis, apoptosis and tumor suppression. It plays a crucial role in bone metabolism where is acts as a coupling factor between bone resorption and bone formation. TGFB1 has affinity to TGFB1, 2 and 3 receptors. Downstream TGFB1 signaling activates Smad proteins, mitogen-activated protein kinases and protein kinase B. Mutations in the gene can result in Camurati-Engelmann disease (characterized by increased bone density). Numerous cancer cells are also known to secrete increased levels of TGFB1.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

ELH-TGFb1-1 was used to measure TGF-β levels in apical and basolateral secretions from mock- and measles virus–infected human airway epithelial cultures at 3 days post-infection. Figure adapted from Hippee et al., J Virol, 2025.

ELH-TGFb1 was used to measure TGF-β1 levels in conditioned media from stromal fibroblasts and prostate cancer cell co-culture experiments. Figure adapted from Pu et al., Sci Adv., 2024.

ELH-TGFb1 was used to measure TGF-β1 levels in conditioned media from oral squamous cell carcinoma cells following EZH2 inhibition. Figure adapted from Campolo et al., Pharmaceuticals (Basel), 2024.

ELH-TGFb1 was used to measure TGF-β in conditioned media from CD4+ T lymphocytes co-cultured with cytokine-treated human periodontal ligament-derived mesenchymal stromal cells. Figure adapted from Behm et al., Frontiers in Immunology, 2023.

ELH-TGFb1 was used to measure TGF-β1 in culture media from RalGAPβ-deficient and control pancreatic ductal adenocarcinoma cell lines. Figure adapted from Cao et al., Journal of Biological Chemistry, 2023.

ELH-TGFb1 and ELH-TGFb2 were used to measure TGF-β1 and TGF-β2 in cervicovaginal lavage samples from women with infertility or habitual abortion. Table adapted from Fernández et al., Nutrients, 2023.

ELH-TGFb1 was used to measure TGFβ in conditioned media from breast cancer stem cells and non-stem cancer cells. Figure adapted from Mukherjee et al., Discover Oncology, 2023.

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate

Additional Materials Required

  • Distilled or deionized water
  • Precision pipettes to deliver 2 µL to 1 µL volumes
  • Adjustable 1-25 µL pipettes for reagent preparation
  • 100 µL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µL of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

documents

Datasheet (ELH-TGFb1.pdf)
SDS (ELH-TGFb1.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Epithelial-to-mesenchymal transition and live cell extrusion contribute to measles virus release from human airway epithelia

    Year:
    2025
    Journal Name:
    Journal of Virology
    PubMed ID:
    39791903

  • Aloin blocks the malignant behavior of lung squamous cell carcinoma cells and M2 macrophage polarization by modulating the NR3C2/MT1M axis

    Year:
    2025
    Journal Name:
    J Integr Med
    PubMed ID:
    40102085

  • Stromal-derived MAOB promotes prostate cancer growth and progression

    Year:
    2024
    Journal Name:
    Sci Adv
    PubMed ID:
    38335292

  • EZH2 Inhibition to Counteract Oral Cancer Progression through Wnt/β-Catenin Pathway Modulation

    Year:
    2024
    Journal Name:
    Pharmaceuticals (Basel)
    PubMed ID:
    39204206

  • 25-hydroxyvitamin D3 generates immunomodulatory plasticity in human periodontal ligament-derived mesenchymal stromal cells that is inflammatory context-dependent

    Species:
    Human
    Sample Type:
    Human periodontal ligament-derived mesenchymal stromal cells (hPDL-MSCs)
    Year:
    2023
    Journal Name:
    Frontiers in immunology

  • Ral GTPase promotes metastasis of pancreatic ductal adenocarcinoma via elevation of TGF-β1 production

    Species:
    Human
    Sample Type:
    MIA PaCa-2 cell, PANC-1 cell
    Year:
    2023
    Journal Name:
    Journal of Biological Chemistry

  • Immunomodulation of the Vaginal Ecosystem by Ligilactobacillus salivarius CECT 30632 Improves Pregnancy Rates among Women with Infertility of Unknown Origin or Habitual Abortions

    Species:
    Human
    Sample Type:
    cervicovaginal lavage (CVL) sample
    Year:
    2023
    Journal Name:
    Nutrients

  • An optimized procedure for preparation of conditioned medium from Wharton’s jelly mesenchymal stromal cells isolated from umbilical cord

    Species:
    Human
    Sample Type:
    mesenchymal stromal cells (MSCs)
    Year:
    2023
    Journal Name:
    Frontiers in molecular biosciences

  • Low vitamin D status is associated with inflammatory response in older patients with cerebral small vessel disease

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Journal of Neuroimmunology

  • Breast cancer stem cells generate immune-suppressive T regulatory cells by secreting TGFβ to evade immune-elimination

    Year:
    2023
    Journal Name:
    Discover oncology
    PubMed ID:
    38038865

You might also be interested by the following products:



Cat#
Description
Cond.
Price Bef. VAT
6511-SC
Corneal Epithelial Cell Medium
 500ml