IL-2, Recombinant, Mouse, Animal Free (T-Cell Growth Factor, TCGF, Interleukin 2)

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes.

Recombinant protein corresponding to mouse IL-2 containing 148 amino acid residues, expressed in E. coli. Accession/Uniprot: P04351

Amino Acid Sequence:
PTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ

Biological Activity:
The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is ≤ 0.2ng/ml, corresponding to a specific activity of ≥5x10e6 units/mg.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.