Immunoglobulin G2 (Biotin)

There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). Human IgG2 subclass has been identified to exist as an ensemble of distinct isoforms, designated IgG2-A, IgG2-B, and IgG2-A/B, which differ by the disulfide connectivity at the hinge region. IgG2, the second largest part of IgG isotypes in humans, comprises 20-25% of the main subclass and is the prevalent immune response against carbohydrate-/polysaccharid antigens.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.2-2ug/ml
Immunohistochemistry: 5-20ug/ml
Immunocytochemistry: 5-20ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.