General Information
| Short Description | This bFGF/FGF2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen | Capture immunogen: Recombinant Mouse bFGF/FGF2 protien.Detection Immunogen: Recombinant Mouse bFGF/FGF2 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. |
| Function | Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation. |
| Protein Name | Fibroblast Growth Factor 2Fgf-2Basic Fibroblast Growth FactorBfgfHeparin-Binding Growth Factor 2Hbgf-2 |
| Database Links | |
| Cellular Localisation | SecretedNucleusExported From Cells By An Endoplasmic Reticulum (Er)/Golgi-Independent MechanismUnconventional Secretion Of Fgf2 Occurs By Direct Translocation Across The Plasma MembraneBinding Of Exogenous Fgf2 To Fgfr Facilitates Endocytosis Followed By Translocation Of Fgf2 Across Endosomal Membrane Into The CytosolNuclear Import From The Cytosol Requires The Classical Nuclear Import MachineryInvolving Proteins Kpna1 And Kpnb1As Well As Cep57 |
| Alternative Antibody Pair Names | Fibroblast Growth Factor 2 antibody pairFgf-2 antibody pairBasic Fibroblast Growth Factor antibody pairBfgf antibody pairHeparin-Binding Growth Factor 2 antibody pairHbgf-2 antibody pairFGF2 antibody pairFGFB antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse bFGF/FGF2 (Fibroblast Growth Factor 2) Antibody Pair (STJAP00556)
This antibody pair is designed to detect mouse bFGF/FGF2 (Fibroblast Growth Factor 2), with reactivity confirmed for mouse. Both the capture and detection antibodies were raised against recombinant mouse bFGF/FGF2 protein (UniProt P09038).
The capture antibody is supplied unconjugated at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol (pH 7.5), purified by Protein A or G. The detection antibody is biotin-conjugated at 0.5 mg/mL in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol (pH 7.5), also purified by Protein A or G.
This antibody pair has been validated for use in ELISA. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
This antibody pair is suitable for use with serum, plasma, and other biological fluids of mouse origin.
Both the capture and detection antibodies should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Both the capture and detection antibodies are mouse-derived, which may introduce background signal when working with mouse sample matrices due to cross-reactivity with endogenous mouse immunoglobulins. Researchers should include appropriate controls and may wish to use anti-mouse immunoglobulin blocking reagents to minimize potential interference.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.