Neo Trypsin-EDTA (0.05 %) in DPBS (1x)
Cat# NB-58-0108
Size : 100ml
Brand : Neo Biotech
Contact local distributor :
Phone : +1 850 650 7790
Description
Neo Trypsin-EDTA (0.05%) in DPBS (1x) combines porcine pancreas-derived trypsin with EDTA for detaching adherent cells from culture surfaces.
Trypsin requirements vary with cell type and culture age. Compare formulations to select suitable conditions for each application.
Product Features
- Cell detachment and dissociation.
- 0.05% trypsin in 1x DPBS.
- Sterile-filtered, ready-to-use frozen solution.
- 100 mL format.
Product Specifications
| Property | Details |
|---|---|
| Appearance | Clear frozen liquid |
| Volume | 100 mL |
| Origin | Porcine pancreas |
| Sterility | Sterile-filtered |
| Storage | ≤ −15°C; avoid repeated freezing and thawing; aliquot. |
| After Opening | +4°C; use within 2–4 weeks. |
| Shipping | Frozen on dry ice |
| Thawing | 37°C water bath or overnight at 2–8°C; gently swirl. |
| Intended Use | Research and further manufacturing only |
Formulation
| Component | Concentration (mg/L) |
|---|---|
| EDTA 2Na | 220 |
| KCl | 200 |
| KH2PO4 | 200 |
| NaCl | 8,000 |
| Na2HPO4 | 1,150 |
| Trypsin | 500 |
Cell Detachment Procedure
Work aseptically in a laminar flow hood.
1. Remove medium; rinse with calcium- and magnesium-free salt solution; aspirate.
2. Prewarm reagent to 37°C; cover cells.
3. Incubate at 37°C; sensitive cultures: room temperature or 2–8°C.
4. Monitor rounding and cloudiness frequently; minimize exposure to prevent damage.
5. Neutralize with serum-containing medium or trypsin inhibitor; gently centrifuge; discard supernatant.
6. Resuspend in fresh medium; count or culture.
Detachment time varies with cell type, culture age, density, serum concentration, and time since subculture.