Rabbit Anti-FABP1 Polyclonal Antibody

Cat# HPA028275

Size : 100ul

Brand : Atlas Antibodies

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Phone : +1 850 650 7790

HPA028275
fatty acid binding protein 1, liver

Anti-FABP1 Antibody

Polyclonal Antibody against HUMAN FABP1

0.1 mg/ml
info@biovalley.fr

Immunohistochemical staining of human kidney shows weak cytoplasmic positivity in cells in tubules.

Product details

  • Target protein: fatty acid binding protein 1, liver
  • Target gene: FABP1
  • Verified species reactivity: Human, Mouse, Rat
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000054422 (84%)RAT - ENSRNOG00000006675 (83%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000047592
  • Concentration: 0.1 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:2500 - 1:5000
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC) Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. 
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: fatty acid binding protein 1, liver
  • Gene name: FABP1
  • Alternative gene names: L-FABP
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (7)

  • Chia S, Wen Seow JJ, Peres da Silva R, Suphavilai C, Shirgaonkar N, Murata-Hori M, Zhang X, Yong EY, Pan J, Thangavelu MT, Periyasamy G, Yap A, Anand P, Muliaditan D, Chan YS, Siyu W, Yong CW, Hong N, Ran G, Sim NL, Guo YA, Yi Teh AX, Wei Ling CC, Wei Tan EK, Pei Cherylin FW, Chang M, Han S, Seow-En I, Chen Hui LR, Hsia Gan AH, Yap CK, Ng HH, Skanderup AJ, Chinswangwatanakul V, Riansuwan W, Trakarnsanga A, Pithukpakorn M, Tanjak P, Chaiboonchoe A, Park D, Kim DK, Iyer NG, Tsantoulis P, Tejpar S, Kim JE, Kim TI, Sampattavanich S, Tan IB, Nagarajan N, DasGupta RCell Rep Med , 2025 Apr 4; 6(4):102053. Epub 2025 Apr 42025 Apr 4

  • Gupta T, Antanaviciute A, Hyun-Jung Lee C, Ottakandathil Babu R, Aulicino A, Christoforidou Z, Siejka-Zielinska P, O'Brien-Ball C, Chen H, Fawkner-Corbett D, Geros AS, Bridges E, McGregor C, Cianci N, Fryer E, Alham NK, Jagielowicz M, Santos AM, Fellermeyer M, Davis SJ, Parikh K, Cheung V, Al-Hillawi L, Sasson S, Slevin S, Brain O, TIP Consortium, Fernandes RA, Koohy H, Simmons ACancer Cell , 2024 May 13; 42(5):797-814.e152024 May 13

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