Orb1179085-100ug | Recombinant IFN gamma (Interferon gamma), Human

Recombinant IFN gamma (Interferon gamma), Human, Animal-Free

SKU: orb1179085

Recombinant IFN gamma (Interferon gamma), Human, Animal-Free

Infectious Disease & Virology; Immunology & Inflammation

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Tested Applications	Cell Culture, ELISA

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Source	Escherichia coli
Expression System	Escherichia coli
Biological Origin	Human
Biological Activity	Measure by its ability to induce cytotoxicity in HT29 cells. The ED50 for this effect is <0.5 ng/mL. The specific activity of recombinant human IFN gamma is approximately >1.3 x 107 IU/mg.
Reactivity	Human
Tag	His-tag at the N-terminus
Endotoxins	<0.01 EU per 1 μg of the protein by the LAL method.
Purity	>95% as determined by SDS-PAGE. Ni-NTA chromatography.
Protein Sequence	MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFQGRRASQ with polyhistidine tag at the Cterminus.

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Storage	Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Form/Appearance	Lyophilized
Buffer/Preservatives	The protein was lyophilized from a solution containing 1X PBS, pH 8.0.
Reconstitution	It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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Type II interferon, T-cell interferon, MAF

NCBI Gene ID

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Failed to load NCBI Gene data

Protein Handling and Storage Guide

Protein Handling Guide

ELISA

Enzyme-linked Immunosorbent Assay (EIA)