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> Recombinant SARS-CoV-2 Spike Protein, S1 Subunit

Recombinant SARS-CoV-2 Spike Protein, S1 Subunit

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Cat# 230-01101-100

Size : 100ug

Brand : RayBiotech

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Phone : +1 850 650 7790

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Recombinant SARS-CoV-2 Spike Protein, S1 SubunitRayBiotech's SARS-CoV-2 S1 protein induced IL-6 expression, IL-6 secretion, and STAT3 phosphorylation in brain endothelial cells. Figure adapted from Stangis et al., NeuroImmune Pharmacol. Ther., 2024.Figure adapted from Atanasova et al., Pharmaceuticals, 2024.Figure adapted from Ponce-de-León et al., npj Vaccines, 2023.RayBiotech's SARS-CoV-2 S1 protein was used to stimulate endothelial cells, triggering inflammatory responses including IL-6 (shown here) and MCP-1 upregulation. Figure adapted from Montezano et al., Scientific Reports, 2023.Figure adapted from Bejoy et al., Arch Biochem Biophys, 2023.

Recombinant SARS-CoV-2 Spike Protein, S1 Subunit

Recombinant SARS-CoV-2 Spike Protein, S1 Subunit

Recombinant SARS-CoV-2 Spike Protein, S1 Subunit derived from E.coli
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Product Description

Specifications

Size100 µg
SpeciesSARS-CoV-2
Accession Number
QHD43416
Protein Name / Synonyms
SARS-CoV-2 Spike Protein, S1 Subunit, S protein, spike protein
Expressed Region
Val16 - Gln690
Expression SystemE. coli
TagN-terminal histidine tag
Purity
>80 %
Purity Determined BySDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)
~75 kDa
Format
Liquid
Formulation
Filtered solution in 50 mM Tris-HCl, 500 mM NaCl, 600 mM arginine, 2 mM oxidized glutathione, 4 mM reduced glutathione, 0.2% PEG4000, 10% glycerol, 2 mM DTT, 20 mM Hepes (pH 9).
Concentration (lot specific)
Lot specific (see the label on the vial), determined by Bradford protein assay.
Research AreaInfectious Disease, COVID-19
Shipping TypeBlue ice
Storage-20°C
SDS-PAGE Image
SDS-PAGE
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Introduction

The SARS-CoV-2 spike (S) protein is a surface glycoprotein that mediates viral entry into host cells. The S1 subunit contains the receptor-binding domain (RBD), which is responsible for recognizing and binding to the ACE2 receptor. This interaction is the first step in viral infection, making the S1 subunit a critical focus for vaccine development and antiviral therapies.

Reference: https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03143-7

Typical Data

RayBiotech's SARS-CoV-2 S1 protein (Cat# 230-01101) induced IL-6 expression, IL-6 secretion, and STAT3 phosphorylation in brain endothelial cells. Figure adapted from Stangis et al., NeuroImmunePharmacol. Ther., 2024.

RayBiotech's SARS-CoV-2 S1 protein (Cat# 230-01101) was used as an ELISA antigen to evaluate vaccine-induced IgG responses in a multi-epitope immunization study. The study also included our S2 protein (Cat# 230-01103), along with additional RayBiotech viral antigens, to assess broader humoral immunity. Figure adapted from Atanasova et al., Pharmaceuticals, 2024.

RayBiotech's SARS-CoV-2 S1 protein (Cat# 230-01101) was used to coat ELISA plates for detecting spike-specific IgG responses in a phase I COVID-19 vaccine trial. Shown here: IgG levels after high-dose intramuscular vaccination. Figure adapted from Ponce-de-León et al., npj Vaccines, 2023.

RayBiotech's SARS-CoV-2 S1 protein (Cat# 230-01101) was used to stimulate endothelial cells, triggering inflammatory responses including IL-6 (shown here) and MCP-1 upregulation. Figure adapted from Montezano et al., Scientific Reports, 2023.

RayBiotech’s recombinant spike proteins and binding assay kit were used to assess ACE2-binding inhibition by viral spike domains. Shown here: inhibition of ACE2–RBD binding by SARS-CoV-2 S1 (Cat# 230-01101), SARS-CoV-2 RBD (Cat# 230-01102), and other viral proteins using the Spike–ACE2 Binding Assay Kit I (Cat# CoV-SACE2). Figure adapted from Bejoy et al., Arch BiochemBiophys, 2023.

citations

  • Spike 1 protein of SARS-CoV-2 induces endothelial inflammation and vascular dysfunction through interferon ISG15-dependent mechanisms

    Species:
    Human
    Sample Type:
    Tissue Lysate / Homogenate (endothelial cells)
    Year:
    2026
    Journal Name:
    Cardiovascular Research
    PubMed ID:
    42150851

  • An exploratory effect of Phaleria macrocarpa fruit extract on interleukin-6 mRNA expression in human PBMCs induced by glucose-enhanced SARS-CoV-2 spike protein and lipopolysaccharide in vitro

    Species:
    Human
    Sample Type:
    Other / Unspecified (adherent PBMCs)
    Year:
    2026
    Journal Name:
    Medicni perspektivi

  • Recombinant SARS-Cov-2 spike protein S1 subunit upregulates P2 × 7-NLRP3 leading to macrophage pyroptosis promoting acute lung injury

    Species:
    Mouse
    Sample Type:
    Other / Unspecified (K18-hACE2 mice tracheal instillation)
    Year:
    2026
    Journal Name:
    Respiratory Research
    PubMed ID:
    41572306

  • SARS-CoV-2 Spike Protein Stimulates Macropinocytosis in Murine and Human Macrophages via PKC-NADPH Oxidase Signaling

    Species:
    Mouse
    Sample Type:
    Other / Unspecified (human macrophages)
    Year:
    2024
    Journal Name:
    Antioxidants
    PubMed ID:
    38397773

  • HU308, A Selective Cannabinoid Type-2 Receptor Agonist, Mitigates SARS-CoV-2 Spike Protein-Induced Acute Lung Injury in Mice

    Species:
    Mouse
    Sample Type:
    Other / Unspecified (mice intratracheal injection, in vivo)
    Year:
    2026
    Journal Name:
    Lung
    PubMed ID:
    41656368

  • Differential Cytokine Responses of APOE3 and APOE4 Blood-brain Barrier Cell Types to SARS-CoV-2 Spike Proteins

    Year:
    2024
    Journal Name:
    J Neuroimmune Pharmacol
    PubMed ID:
    38771543

  • A fast and easy one-step purification strategy for plant-made antibodies using Protein A magnetic beads

    Year:
    2024
    Journal Name:
    Frontiers in plant science
    PubMed ID:
    38235198

  • Design, Development and Immunogenicity Study of a Multi-Epitope Vaccine Prototype Against SARS-CoV-2

    Year:
    2024
    Journal Name:
    Pharmaceuticals (Basel)
    PubMed ID:
    39598409

  • Interim safety and immunogenicity results from an NDV-based COVID-19 vaccine phase I trial in Mexico

    Year:
    2023
    Journal Name:
    NPJ vaccines

  • Recombinant human plasma gelsolin reverses increased permeability of the blood-brain barrier induced by the spike protein of the SARS-CoV-2 virus

    Year:
    2022
    Journal Name:
    Journal of Neuroinflammation
    PubMed ID:
    36434734

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