Orb348556 | Ripa Lysis Buffer Biovalley

Ripa Lysis Buffer

SKU: orb348556

Description

Ripa Lysis Buffer

Images & Validation

−

Application Notes	This product is ready-to-use as a working 1X solution and requires no further dilution. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein immunoreactivity and biological activity. We recommend using 1.0 ml of RIPA Lysis Buffer to lyse 0.5 to 5 x 10E7 adherent mammalian cells. This buffer contains ionic detergents and may not be suitable for kinase enzymes, if these enzymes are easily denatured. Do not add phosphatase inhibitors when preparing lysates for phosphatase assays. 1X RIPA lysis buffer consists of 50 mM Tris HCl, 150 mM NaCl, 1.0% (v/v) NP-40, 0.5% (w/v) Sodium Deoxycholate, 1.0 mM EDTA, 0.1% (w/v) SDS and 0.01% (w/v) sodium azide at a pH of 7.4. This buffer was meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water. Protease and phosphatase inhibitors are recommended but not included in product composition. Recommended final concentrations of protease inhibitors: 1.0 mM Phenylmethylsulfonyl fluoride (PMSF) 10 μM Leupeptin 0.1 μM Aprotinin 1.0 μM Pepstatin Recommended final concentrations of phosphatase inhibitors: 1.0 mM Na3VO4 1.0 mM NaF

Application Notes

This product is ready-to-use as a working 1X solution and requires no further dilution. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein immunoreactivity and biological activity. We recommend using 1.0 ml of RIPA Lysis Buffer to lyse 0.5 to 5 x 10E7 adherent mammalian cells. This buffer contains ionic detergents and may not be suitable for kinase enzymes, if these enzymes are easily denatured. Do not add phosphatase inhibitors when preparing lysates for phosphatase assays. 1X RIPA lysis buffer consists of 50 mM Tris HCl, 150 mM NaCl, 1.0% (v/v) NP-40, 0.5% (w/v) Sodium Deoxycholate, 1.0 mM EDTA, 0.1% (w/v) SDS and 0.01% (w/v) sodium azide at a pH of 7.4. This buffer was meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water. Protease and phosphatase inhibitors are recommended but not included in product composition. Recommended final concentrations of protease inhibitors: 1.0 mM Phenylmethylsulfonyl fluoride (PMSF) 10 μM Leupeptin 0.1 μM Aprotinin 1.0 μM Pepstatin Recommended final concentrations of phosphatase inhibitors: 1.0 mM Na3VO4 1.0 mM NaF

Key Properties

−

Purity	1X RIPA Lysis Buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Store container at room temperature (18° to 26° C) prior to opening. Protect from light (store in the dark).
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: See application note.
Concentration	1X
Expiration Date	12 months from date of receipt.
Hazard Information	Non-Toxic
Disclaimer	For research use only

Alternative Names

−

1X RIPA Lysis Buffer, 1X RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer, RIPA Buffer

−

10X RIPA Lysis Buffer [orb420112]
Applications:
ChIP, IP, WB
Sizes Available: 15 ml
RadioImmunoPrecipitation Assay (RIPA) Buffer, 10X [orb2652890]
Applications:
IHC
Sizes Available: 500 ml
RadioImmunoPrecipitation Assay (RIPA) Buffer, 10X [orb2652891]
Applications:
IHC
Sizes Available: 100 ml
Ripa Lysis Buffer [orb348557]
Applications:
ChIP, IP, WB
Sizes Available: 5 x 50 ml
RIPA Lysis Buffer (Weak) [orb1962289]
Sizes Available: 100 ml