Rotavirus (RV) antigen (Ag) BioAssay™ ELISA Kit (Equine)

Sample Type:
Feces

Intended Use:
For the qualitative determination of equine rotavirus (RV) antigen (Ag) concentrations in feces.

Test Principle:
This BioAssay™ employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rotavirus antigen. Samples are pipetted into the wells with antibody conjugated Horseradish Peroxidase (HRP). Any Rotavirus antigen present in samples will bind to the pre-coated specific antibody. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of horse rotavirus (RV) antigen (Ag) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Specificity:
This assay has high sensitivity and excellent specificity for detection of equine rotavirus (RV) antigen (Ag). No significant cross-reactivity or interference between equine rotavirus (RV) antigen (Ag) and analogues was observed.

Kit Components:
Assay plate, 1x96 wells
Negative Control, 1x800ul
Positive Control, 1x800ul
HRP-conjugate, 1x6ml
Wash Buffer, 1x6ml
Substrate A, 1x5ml
Substrate B, 1x5ml
Stop Solution, 1x5ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.