T4 Polynucleotide Kinase
Cat# MBS9141652-250U
Size : 250Units
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
T4 Polynucleotide Kinase Enzyme
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9141652SPECIAL PROMOTION: Get FREE Amazon gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the gamma position of ATP to the 5' -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3'-monophosphates. T4 Polynucleotide Kinase also… More details
Product Overview
Online Datasheet in PDFProduct Name
T4 Polynucleotide Kinase, Enzyme
Full Product Name
T4 Polynucleotide Kinase
Host
E Coli
Form/Format
10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1 uM ATP pH7.4 at 25 degree C
Concentration
10,000 U/ml (lot specific)
Preparation and Storage
Store at -20 degree C.
Source Note
An E Coli strain that carries the cloned T4 Polynucleotide Kinase gene.
Unit Definition
One Richardson unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [32P] in a total reaction volume of 50 mul in 30 minutes at 37 degree C in 1X T4 PNK Reaction Buffer with 66 uM [gamma-32>P] ATP (5 x 106 cpm/umol) and 0.26 mM 5'-hydroxyl-terminated salmon sperm DNA.
Reaction Conditions
1X T4 PNK Reaction Buffer, Incubate at 37 degree C
Reaction Buffer or Mix
1X T4 PNK Reaction Buffer:
70 mM Tris-HCl, 10 mM MgCl2, 5 mM DTT, pH7.6 at 25 degree C
70 mM Tris-HCl, 10 mM MgCl2, 5 mM DTT, pH7.6 at 25 degree C
Heat Inactivation
65 degree C for 20 min.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for T4 Polynucleotide Kinase enzyme
Phosphorylation (Kinase)
Other Notes
Small volumes of T4 Polynucleotide Kinase enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for T4 Polynucleotide Kinase purchase
MBS9141652 is an enzyme targeting T4 Polynucleotide Kinase. Host: E Coli.
Additional Details
NCBI/Uniprot data below describe general gene information for T4 Polynucleotide Kinase. It may not necessarily be applicable to this product.
Protein Family
Polyribonucleotide 5'-hydroxyl-kinase
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.