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Reagents and instruments for immunology, cell biology and molecular biology.

Tetrazolium Red [298-96-4]

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Cat# NB-64-09428-5g

Size : 5g

Contact local distributor :

Phone : +1 850 650 7790

Brand : Neo Biotech

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Tetrazolium Red

(Synonyms: TTC, TPTZ, 2,3,5-Triphenyltetrazolium chloride) Copy Product Info

Synonyms: TTC, TPTZ, 2,3,5-Triphenyltetrazolium chloride

Catalog No. T19577 Copy Product Info

Purity: 99.82%

Tetrazolium Red (2,3,5-Triphenyltetrazolium chloride) is employed to visualize dehydrogenase enzyme activity.

Cas No. 298-96-4

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.82%

Appearance:Solid

Color:White to Yellow

Product Introduction

Tetrazolium Red AI Summary

Tetrazolium Red demonstrates significant antimalarial activity against Plasmodium falciparum with an IC50 value of 300.0 nM and exhibits toxicity in CHO cells with an IC50 value of 71000.0 nM, providing a selectivity ratio of 236.7 favoring antimalarial action. It shows inhibitory effects on various biological targets including nuclear factor erythroid 2-related factor 2 (Nrf2) with an IC50 of 20596.2 nM, transforming growth factor-b (TGF-b) with IC50s ranging from 10000.0 to 11220.2 nM, and the Marburg Virus with an IC50 of 28183.8 nM. Additionally, Tetrazolium Red inhibits growth in various human tumor cell lines with GI50 values spanning 184.08 nM to 100000.0 nM, including renal, breast, lung, leukemia, prostate, central nervous system, melanoma, ovarian, and colon cell lines. The compound further inhibits activities related to ClpP activation, hepatitis C virus (HCV), ATXN expression, HIV-1 protein Vpr, synthetic lethality in tumor cells producing 2HG, and human tyrosyl-DNA phosphodiesterase 1 (TDP1) with diverse potencies. It has a minimal effect on the displacing ability of SYBR green I from salmon testes DNA, with an IC50 greater than 2500000.0 nM. In terms of antimicrobial properties, Tetrazolium Red shows limited antibacterial activity against Klebsiella pneumoniae and Pseudomonas aeruginosa with inhibition percentages of 5.91% and 16.55%, respectively. It demonstrates minor antifungal activity against Candida albicans with an inhibition percentage of 0.89% but no activity against Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli, and Cryptococcus neoformans. Overall, Tetrazolium Red displays a broad spectrum of bioactivities with notable antimalarial and tumor growth inhibitory effects..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Tetrazolium Red (2,3,5-Triphenyltetrazolium chloride) is employed to visualize dehydrogenase enzyme activity.
Synonyms	TTC, TPTZ, 2,3,5-Triphenyltetrazolium chloride

Chemical Properties

Molecular Weight	334.8
Formula	C₁₉H₁₅ClN₄
Cas No.	298-96-4
Smiles	[Cl-].c1ccc(cc1)-c1nn(-c2ccccc2)[n+](n1)-c1ccccc1
Relative Density.	1.1704 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Keep away from direct sunlight,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 252.5 mg/mL (754.18 mM), Sonication is recommended.

H2O: 50 mg/mL (149.34 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 1.5 mg/mL (4.48 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9869 mL	14.9343 mL	29.8686 mL	149.3429 mL
5 mM	0.5974 mL	2.9869 mL	5.9737 mL	29.8686 mL
10 mM	0.2987 mL	1.4934 mL	2.9869 mL	14.9343 mL
20 mM	0.1493 mL	0.7467 mL	1.4934 mL	7.4671 mL
50 mM	0.0597 mL	0.2987 mL	0.5974 mL	2.9869 mL
100 mM	0.0299 mL	0.1493 mL	0.2987 mL	1.4934 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

Tetrazolium RedInhibitorinhibit2,3,5-Triphenyltetrazolium Chloride2,3,5-Triphenyltetrazolium

Related Tags: Tetrazolium Red chemical structure | Tetrazolium Red formula | Tetrazolium Red molecular weight

Tetrazolium Red [298-96-4]

Cat# NB-64-09428-5g

Contact local distributor :

Brand : Neo Biotech

Contact local distributor :

Tetrazolium Red

Product Introduction

Calculator

In Vivo Formulation Calculator (Clear solution)

Dose Conversion

Keywords

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