Free Western Blot Trial Kit+ 50% Discount on Components!
Western blotting is a widely used laboratory technique for detecting and quantifying specific proteins in complex biological samples. The method typically involves protein separation by SDS-PAGE , transfer to a membrane , and antibody-based detection of target proteins. Detection often relies on enzymatic reactions that produce a measurable signal, such as chemiluminescence.
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Explore the Kit's Components
In Western blotting, primary antibodies detect target proteins on the membrane, with housekeeping proteins like GAPDH and β-Actin serving as loading controls. An HRP-conjugated secondary antibody reacts with an HRP substrate to produce a luminescent signal, while protein ladders provide molecular weight markers for accurate size estimation.
Whole cell extracts blotted with GAPDH antibody [GT239] (GTX627408) .
- Monoclonal antibody specific for GAPDH .
- Validated for Western blot applications .
- Successfully detects GAPDH in various whole cell extracts .
- Compatible with SDS-PAGE and detection using a secondary antibody .
Anti-beta Actin Antibody [HL1926]
V arious whole cell extracts blotted with beta Actin antibody [HL1926] (GTX637675) .
- Rabbit monoclonal antibody targeting β-actin .
- Commonly used as a loading control in Western blotting.
- Provides reliable detection in Western blot assays.
- Compatible with HRP-conjugated secondary antibodies for signal detection.
Trident femto Western HRP substrates
Recombinant mCherry was detected using an mCherry antibody, followed by an HRP-conjugated secondary antibody and Trident femto HRP substrate (GTX14698) .
- Chemiluminescent substrate system for HRP detection.
- Designed for high sensitivity , detecting HRP activity down to the femtogram level .
- Composed of two solutions mixed before use .
- Suitable for detecting very low abundance proteins.
Trident Prestained Protein Ladder
Migration pattern of GTX49384 Trident Prestained Protein Ladder (Standard Range) in Tris-Glycine gel.
- Prestained protein ladder with covalently attached colored chromophores.
- Covers a broad range of molecular weights (~10–180 kDa in standard buffers).
- Enables real-time monitoring of electrophoresis .
- Allows assessment of protein transfer to membranes .