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Deletion of DE prophage from E. coli expression strain

Strain modifications examples

 

Using Red/ET recombination 89%of DE prophage sequence was removed from the genome of the E. coli expression strain BL21(DE3). The optimized strain T7E1 shows no phage activity while exhibiting consistent bacterial growth and heterologous protein expression pattern compared to the wildtype.

Voir tous les Kits de recombinaison Red/ET :
redet-kit

prophage

 

Noll S, Reyelt J, Rysiok T, Kellner, R, Güssow D, Jäkel S, Hager S and Kranz H, 2013, Gezielte Optimierung von Escherichia coli BL21(DE3), Biospektrum, 19, 211