Images
QC Test

A calcium standard curve is shown with 96-well black plate and the Calcium Assay Kit (Red Fluorescence) (Cat # KA4080). As low as 0.01 mM calcium can be detected with 5 minutes incubation time (n=3). The fluorescence in blank wells (with H2O only) is used as a control, and is subtracted from the values for those wells with calcium reactions. Note: The fluorescence background increases with time, thus it is important to subtract the fluorescence intensity value of the blank wells for each data point.

  • Specification

    Product Description

    Calcium Assay Kit (Red Fluorescence) is used for the measurement of calcium level in physiology solutions.

    Suitable Sample

    Physiological Solutions

    Sample Volume

    50 uL

    Excitation/<br>Emission (nm)

    540/590 nm

    Detection Method

    Fluorometric

    Instrument Platform

    Microplate Reader

    Calibration Range

    0.003 to 3 mM

    Limit of Detection

    0.03 mM

    Regulation Status

    For research use only (RUO)

    Storage Instruction

    Store the kit in desiccated environment at -20°C and avoid from light.

  • Applications

    Quantification