EnzyFluo™ Isocitrate Assay Kit

Référence EFIC-100

Conditionnement : 100tests

Marque : BioAssay Systems

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EnzyFluo™ Isocitrate Assay Kit



Application

  • For quantitative fluorimetric determination of isocitrate and evaluation of drug effects on its metabolism.

Key Features

  • Fast and sensitive. Linear detection range (20 µL sample): 0.6 to 500 µM for 10 min reaction.
  • Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

  • FL530/585 nm

Samples

  • Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)

Species

  • All

Procedure

  • 10 min

Size

  • 100 tests

Detection Limit

  • 0.6 µM

Shelf Life

  • 6 months

More Details

ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. BioAssay Systems’ isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the isocitrate concentration in the sample.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Isocitrate Assay Kit
Catalog No: EFIC-100 Categories Agriculture & Environment, Diabetes & Obesity, Food & Beverage Analysis, Metabolism Tags Agriculture & Environment, Diabetes & Obesity, EFIC-100, EnzyFluo Isocitrate Assay Kits, EnzyFluoTM Isocitrate Assay Kit, Food & Beverage Analysis, Isocitrate assay kits, Isocitrate determination kits, Isocitrate measurement kits, Isocitrate test kits, metabolism