L-Lactate Assay Kit, BioAssay™, Colorimetric

Référence L1011-04A-96T

Conditionnement : 96Tests

Marque : US Biological

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Téléphone : +1 850 650 7790


Shipping Temp
Dry Ice

Storage Temp
-20°C

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.

Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. Lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 56 nm, is proportionate to the lactate concentration in the sample.

Applications:
Direct Assays: Lactate in serum, plasma, and cell media samples.

Key Features:
Sensitive and accurate
Detection limit:
96-well plate assay: 0.05mM and linearity up to 2mM L-Lactate.
For cell culture samples containing phenol red: 0.1mM and linearity up to 1mM L-Lactate in 96-well plate assay.

Convenient: The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 minutes. Room temperature assay. No 37°C heater is needed.

High-throughput: Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Kit Components: (100 tests in 96-well plates):
L1011-04A1: Assay Buffer, 1x10ml
L1011-04A2: NAD Solution, 1x1ml
L1011-04A3: Enzyme A, 1x120ul
L1011-04A4: Enzyme B, 1x120ul
L1011-04A5: MTT Solution, 1x1.5ml
L1011-04A6: Standard, 1x1ml Supplied in 20mM L-Lactate

Storage and Stability:
Store components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Precautions:
Reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.