SIRT5 (Sirtuin5) Fluorogenic BioAssay™ Kit

The Fluorogenic SIRT5 BioAssay™ Kit is a complete assay system designed to measure SIRT5 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT5 activity measurements. In addition, the kit includes purified SIRT5 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT5 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only three simple steps on a microtiter plate are needed to analyze the SIRT5 activity level. First, the Sirtuin 5 fluorometric substrate (SIRT substrate 5), containing a succinylated lysine side chain, is incubated with purified SIRT5 enzyme. The desuccinylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

SIRT5 human recombinant enzyme, 1x50ug
Fluorogenic SIRT5 substrate (5mM), 1x50ul
Nicotinamide Adenine Dinucleotide (NAD+) (50mM), 1x50ul
Nicotinamide (10mM), 1x500ul
2x SIRT Developer (contains 2mM Nicotinamide), 1x6ml
SIRT assay buffer, 1x10ml
black, low binding NUNC black microtiter plate

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.