Galanthus nivalis (Snowdrop bulb) (GNA)

Référence G1044-5mg

Conditionnement : 5mg

Marque : US Biological

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Grade
Molecular Biology Grade

Applications
IHC

Shipping Temp
Blue Ice

Storage Temp
-20°C

Snowdrop bulb
Galanthus nivalis agglutinin (GNA) is a small molecular weight tetramer consisting of subunits of about 13,000D. This lectin contains little or no carbohydrate. Unlike most mannose-specific lectins, it is not a metalloprotein and does not require Ca++ or Mn++ for binding. Binding seems to be preferentially directed toward structures containing (a-1,3) mannose residues. In contrast to most mannose-binding lectins, GNA will not bind alpha linked glucose. Reports indicate that this lectin binds IgM, but not bind IgG immunoglobulin classes of rat and mouse. The only protein from human serum reported to bind to this lectin is a2-macroglobulin.

Isoelectric Point: < pH 4.6

Activity (unconjugated): ≤ 15ug/ml will agglutinate human type O erythrocytes.

Carbohydrate Specificity: Mannose

Inhibitory Carbohydrate:
Mannose a-1,3-mannose
Mannose (> 0.2M )

Inhibiting/Eluting Sugar:
100mM-200mM a-methyl mannoside

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source
Galanthus nivalis (snowdrop bulb)

Purity
Highly purified. Three bands at 26kD, 30kD and 44kD detected by SDS-PAGE.

Concentration
~1mg/ml (after reconstitution)

Form
Supplied as a lyophilized powder from PBS, pH 7.3.

Reconstitute with sterile PBS, 0.1% sodium azide.


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.