A431 Cells, EGF-Stimulated
Référence MBS652596-0,1mL
Conditionnement : 0.1mL
Marque : MyBiosource
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EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-phosphotyrosine (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
A431 Cells, EGF-Stimulated Cell Line
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS652596Cellular protein preparation from A431 cells containing phosphorylated proteins, including the EGF receptor. More details
Product Overview
Online Datasheet in PDFProduct Name
A431 Cells, EGF-Stimulated, Cell Line
Full Product Name
A431 Cells, EGF-Stimulated
Host
Human A431 cells
Form/Format
A431 cells were cultured for 20 minutes in the presence of 50ng/ml EGF. Cells were lysed in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM NaCl, 1mM EDTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstat
Purity/Purification
Lysate
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Matching Pairs
Antibody: 14-3-3, sigma Clone #1.T.28 (MBS6241568)
Positive Control: A431 Cells, EGF-Stimulated (MBS652596)
Positive Control: A431 Cells, EGF-Stimulated (MBS652596)
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Product Categories/Family for A431 Cells, EGF-Stimulated cell line
Cells; Lysates; Plasma; Serum; Tissues; Cells-A431
Applications Tested/Suitable for A431 Cells, EGF-Stimulated cell line
Western Blot (WB)
Application Notes for A431 Cells, EGF-Stimulated cell line
Dilution: Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for immunoblot analysis. This preparation may be used as a positive control for antibodies.
Testing Data of A431 Cells, EGF-Stimulated cell line
EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-phosphotyrosine (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Other Notes
Small volumes of A431 Cells, EGF-Stimulated cell line vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for A431 Cells, EGF-Stimulated purchase
MBS652596 is a cell line targeting A431 Cells, EGF-Stimulated. Host: Human A431 cells.