ACPP siRNA (Rat)
Référence MBS827079-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
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ACPP siRNA – Prostatic Acid Phosphatase Long Isoform (Rat)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827079MBS827079 is an siRNA targeting ACPP. Also known as ACPP siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit ACPP expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
ACPP, siRNA
Full Product Name
ACPP siRNA (Rat)
Product Gene Name
ACPP sirna
Product Synonym Names
Prostatic acid phosphatase; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; Fluoride-resistant acid phosphatase; FRAP; Thiamine monophosphatase; TMPase
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
ACPP siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
48,053 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ACPP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for ACPP sirna
RNA Interference (RNAi)
Other Notes
Small volumes of ACPP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for ACPP. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
prostatic acid phosphatase long isoform
NCBI Official Synonym Full Names
acid phosphatase, prostate
NCBI Official Symbol
Acpp
NCBI Official Synonym Symbols
pap; FRAP; Ppal; 5'-NT; Acpp11; RNACPP11
UniProt Protein Name
Prostatic acid phosphatase
UniProt Synonym Protein Names
5'-nucleotidase (EC:3.1.3.5); 5'-NT; Ecto-5'-nucleotidase; Fluoride-resistant acid phosphatase; FRAP; Thiamine monophosphatase; TMPase
Protein Family
Acyl carrier protein
UniProt Gene Name
Acpp
UniProt Synonym Gene Names
5'-NT; FRAP; TMPase
UniProt Entry Name
PPAP_RAT
NCBI Protein Information
prostatic acid phosphatase
UniProt Comments for ACPP
A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Diseases associated with ACPP sirna
| Disease Name | Pubmed Publications |
|---|---|
| Prostatic Neoplasms Antibodies | >9 publications with ACPP and Prostatic Neoplasms |
| Inflammation Antibodies | >4 publications with ACPP and Inflammation |
| Fibrosis Antibodies | >3 publications with ACPP and Fibrosis |
| Breast Neoplasms Antibodies | >3 publications with ACPP and Breast Neoplasms |
| Necrosis Antibodies | >3 publications with ACPP and Necrosis |
| Hyperplasia Antibodies | >3 publications with ACPP and Hyperplasia |
| Ovarian Neoplasms Antibodies | >2 publications with ACPP and Ovarian Neoplasms |
| Urinary Bladder Neoplasms Antibodies | >2 publications with ACPP and Urinary Bladder Neoplasms |
| Neoplasm Metastasis Antibodies | >2 publications with ACPP and Neoplasm Metastasis |
| Myocardial Infarction Antibodies | >2 publications with ACPP and Myocardial Infarction |
Organs/Tissues associated with ACPP sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Dorsal Root Ganglion Antibodies | >1 publications with ACPP and Dorsal Root Ganglion |
| Embryonic Tissue Antibodies | >1 publications with ACPP and Embryonic Tissue |