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> Angiotensin Converting Enzyme (ACE, CD143) (FITC)

Angiotensin Converting Enzyme (ACE, CD143) (FITC)

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Référence A2295-01D-FITC-100ul

Conditionnement : 100ul

Marque : US Biological

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A2295-01D-FITC Angiotensin Converting Enzyme (ACE, CD143) (FITC)

Clone Type
Monoclonal
Host
mouse
Source
human
Swiss Prot
P22966
Isotype
IgG1
Grade
Purified
Applications
FLISA IC IHC IP WB
Crossreactivity
Fe Hm Hu Mk Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation and FLISA. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: fresh frozen or 4% paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue.
Immunocytochemistry: Reacts with endothelial cell, macrophages (activated) and THP1 cells (monocyte line).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 2Q2314 (9B9)|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified immunoglobulin|Immunogen: Angiotensin-converting enzyme from human lung.|Specificity: Recognizes N-terminal epitope of human Angiotensin-converting enzyme (ACE). Species Crossreactivity: monkey, rat, hamster and feline. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Angiotensin-converting enzyme from human lung.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified immunoglobulin
Specificity
Recognizes N-terminal epitope of human Angiotensin-converting enzyme (ACE). Species Crossreactivity: monkey, rat, hamster and feline.
References
1. Histochemistry (1987) 87:487–490. 2. Hypertension (1995) 25:219–226. 3. J. Clin. Invest. (1995) 96:1823–1833. 4. J. Nucl. Med. (1989) 30:1686–1692. 5. J. Nucl. Med. (1988) 29:1951–1958. 6. Nucl. Med. Biol. (1993) 20:435–441. 7. Lab. Invest. (1991) 64:118–124. 8. J. Nucl. Med. (1994) 35:1358–1365. 9. Int. Immunol. (1994) 6:1153–1160. 10. PNAS.USA (1996) 93:5213–5218. 11. J. Nucl. Med. (1991) 32:453–460. 12. Lung (1992) 170:349–358. 13. J. Immunol. Methods. (1990) 132:263–273. 14. J. Hypertension (1996) 14: 719–727. 15. J. Biol. Chem. (1994) 269:26806–26814. 16. Hypertension (1999) 33:732–739. 17. Petrov, V., et al., J Renin-Angiotensin-Aldosterone System (2000) 1:342–352.