Anti-CD20 Antibody-FITC labled
Référence CFE8504-25
Conditionnement : 25Assays
Marque : Cohesion Biosciences
| Product Name: | Anti-CD20 Antibody-FITC labled |
| Cat No: | CFE8504 |
| Source: | Mouse |
| Reactivity: | H |
| Applications: | IF, FC |
| *Application Key: | |
| H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish | |
| *Species Reactivity Key: | |
| E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference | |
Description:Mouse monoclonal antibody FITC labled to CD20Immunogen:Native purified human CD20.Purification:The antibody was purified by affinity chromatography.Clonality:Monoclonal (clone: BCA/B20)Conjugation:FITCForm:Mouse IgG2a. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.Dilution:10 μl / assayGene Symbol:MS4A1Alternative Names:CD20; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD antigen CD20 Entrez Gene (Human): 931; SwissProt (Human): P11836; Directions for Use:1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).Storage/Stability:Shipped and store at 4°C for one year. Do not freeze.
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