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> Anti-CD66a/e Antibody

Anti-CD66a/e Antibody

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Référence CPA1096-100ul

Conditionnement : 100ul

Marque : Cohesion Biosciences

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Téléphone : +1 850 650 7790

  • Western blot analysis of CD66a/e expression in HUT78 (A), HCT116 (B) whole cell lysates. (Predicted band size: 57; 76 kD; Observed band size: 180 kD)
  • Immunohistochemical analysis of CD66a/e staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of CD66a/e staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Product Name:Anti-CD66a/e Antibody
Cat No:CPA1096
Source:Rabbit
Reactivity:H
Applications:WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to CD66a/e
Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD66a/e. The exact sequence is proprietary.
Purification：The antibody was purified by immunogen affinity chromatography.
Specificity：Recognizes endogenous levels of CD66a/e protein.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200)
Gene Symbol：CEACAM1; CEACAM5
Alternative Names：CEACAM1; BGP; BGP1; Carcinoembryonic antigen-related cell adhesion molecule 1; Biliary glycoprotein 1; BGP-1; CD66a; CEACAM5; CEA; Carcinoembryonic antigen-related cell adhesion molecule 5; Carcinoembryonic antigen; CEA; Meconium antigen 100; CD66e
Entrez Gene (Human)： 634; 1048;
SwissProt (Human)： P13688; P06731;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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