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> Anti-CD73 Antibody (8K319)

Anti-CD73 Antibody (8K319)

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Référence NB-64-122881-100uL

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Anti-CD73 Antibody (8K319)

(Synonyms: NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)) Copy Product Info

Synonyms: NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)

Catalog No. TMAY-01319 Copy Product Info
Anti-CD73 Antibody (8K319) is a Rabbit antibody targeting CD73. Anti-CD73 Antibody (8K319) can be used in ELISA,ICC/IF.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-CD73 Antibody (8K319) is a Rabbit antibody targeting CD73. Anti-CD73 Antibody (8K319) can be used in ELISA,ICC/IF.
SynonymsNTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)
Ig Type
Rabbit IgG
Clone
8K319
Reactivity
Human
Specificity
Human 5'-nucleotidase/CD73/NT5E
No cross-reactivity in ELISA with
Mouse NT5E
Verified Activity
Immunofluorescence staining of NT5E-3 in MCF7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human NT5E-3 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm .
Application
ELISA
ICC/IF
Recommended Dose
ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human CD73 / NT5E (rh CD73 / NT5E; TMPY-01851; NP_002517.1; Met1-Lys547).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research Background5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human CD73 / NT5E protein (TMPY-01851)
Antigen Species
Human
Biology Area
Phosphatases and Regulators
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Keywords

NT-5NT 5CD-73CD 735'-nucleotidase, ecto
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