Anti-CDYL2 Antibody
Référence MBS821304-0,2mL
Conditionnement : 0.2mL
Marque : MyBiosource
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Western blot analysis of CDYL2 expression in CT26 (A), PC12 (B), Hela (C), PC3 (D) whole cell lysates.
Immunohistochemical analysis of CDYL2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CDYL2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
CDYL2 Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821304MBS821304 is an antibody targeting CDYL2. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to CDYL2 More details
Product Overview
Online Datasheet in PDFProduct Name
CDYL2, Polyclonal Antibody
Full Product Name
Anti-CDYL2 Antibody
Product Gene Name
anti-CDYL2 antibody
Product Synonym Names
Chromodomain Y-like protein 2; CDY-like 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of CDYL2 protein.
Preparation and Storage
Upon receipt aliquot and store at -20°C. Avoid freeze/thaw cycles.
Applications Tested/Suitable for anti-CDYL2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-CDYL2 antibody
Western Blot (WB) 1/500 - 1/1000
Immunohistochemistry (IHC) 1/100 - 1/200
Immunofluorescence/Immunocytochemistry (IF/ICC) 1/100 - 1/500
Immunohistochemistry (IHC) 1/100 - 1/200
Immunofluorescence/Immunocytochemistry (IF/ICC) 1/100 - 1/500
Immunohistochemistry (IHC) of anti-CDYL2 antibody
Immunohistochemical analysis of CDYL2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescence (IF) of anti-CDYL2 antibody
Immunofluorescent analysis of CDYL2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Other Notes
Small volumes of anti-CDYL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for CDYL2. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
chromodomain Y-like protein 2
NCBI Official Synonym Full Names
chromodomain protein, Y-like 2
NCBI Official Symbol
CDYL2
NCBI Official Synonym Symbols
PCCP1
UniProt Protein Name
Chromodomain Y-like protein 2
Protein Family
Chromodomain Y-like protein
UniProt Gene Name
CDYL2
UniProt Synonym Gene Names
CDY-like 2
UniProt Entry Name
CDYL2_HUMAN
NCBI Protein Information
chromodomain Y-like protein 2; CDY-like 2; prostate cancer candidate protein 1, PCCP1
UniProt Comments for CDYL2
CDYL2: Interacts (via chromo domain) with histone H3K9me3
Chromosomal Location of Human Ortholog: 16q23.2
Cellular Component: nucleus
Molecular Function: protein binding; catalytic activity; methylated histone residue binding
Biological Process: metabolic process
Chromosomal Location of Human Ortholog: 16q23.2
Cellular Component: nucleus
Molecular Function: protein binding; catalytic activity; methylated histone residue binding
Biological Process: metabolic process
Research Articles on CDYL2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Diseases associated with anti-CDYL2 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Genomic Instability Antibodies | >1 publications with CDYL2 and Genomic Instability |
Organs/Tissues associated with anti-CDYL2 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Testis Antibodies | >1 publications with CDYL2 and Testis |