Anti-Human CD56 (NCAM) [Clone ERIC-1] - Purified in vivo GOLD™ Functional Grade

Mouse IgG1 GOLD, Clone hksp OR Mouse IgG1 GOLD, Clone hksp84

Human Retinoblastoma tumor

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multistep process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 28°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ 70°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 28°C

Mouse AntiHuman CD56 (Clone ERIC1) recognizes Human CD56. This monoclonal antibody was purified using multistep affinity chromatography methods such as Protein A or G depending on the species and isotype. AntiHuman CD56 recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM).¹

AntiHuman CD56 can be used in studies of the neural cell adhesion molecule. This antibody may also be used to study isoforms of NCAM expressed on different tumor types. AntiCD56 (clone ERIC1) works well in western blot for analysis of isoforms expressed. AntiCD56 did not react with any of the leukemias or lymphomas tested.¹

The CD56 (NCAM) antigen is expressed on most neuroectodermal derived cell lines, tissues, and tumors. NCAM is also known to be expressed on some mesodermally derived tissues such as muscle¹ and on natural killer (NK) lymphocytes. The binding of ERIC1 to both normal and neoplastic tissue is lost when tissues are conventionally fixed in formalin and embedded in paraffin. The epitope was preserved when exposed to dehydrating fixatives such as cold acetone (20EC) for 5 min. CD56 is present on 1025% of peripheral blood lymphocytes.

Heparan sulfate

1. Bourne, K. et al. (1991) J. of NeuroOncology 10:111