Anti-Mouse CD366 (Tim-3) [Clone RMT3-23] - APC
Référence T750-100
Conditionnement : 100ug
Marque : Leinco Technologies
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AntiMouse CD366 (Tim3) [Clone RMT323] — APC
AntiMouse CD366 (Tim3) [Clone RMT323] — APC
Product No.: T750
Clone RMT323 Target Tim3 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Havcr2 Isotype Rat IgG2a κ Applications FC |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Immunogen This antibody was produced using recombinant mouse TIM3 Product Concentration 0.2 mg/ml Formulation This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multistep process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling This Allophycocyanin (APC) conjugate is stable when stored at 28°C. Do not freeze. Country of Origin USA Shipping Next Day 28°C Excitation Laser Red Laser (650 nm) RRIDAB_2892981 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for CD366 antibody (clone RMT323) for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? B IHC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone RMT223 recognizes an epitope on mouse Tim3. Background Tim3 antibody, clone RMT323, recognizes T cell immunoglobulin and mucin domaincontaining protein 3 (TIM3, also known as CD366), a TIM family member of immunoregulatory proteins. Tim3 is a 60kDa type I transmembrane protein with an extracellular immunoglobulin and mucinlike domain and a cytoplasmic tyrosine phosphorylation motif. Tim3 is expressed by IFNgproducing Th1 CD4 and Tc1 (cytotoxic) CD8 T cells1, regulatory T cells (Tregs)2, myeloid cells3, NK cells4, and mast cells5. Tim3 is an inhibitory molecule that limits Th1mediated inflammatory diseases, including a model of central nervous system inflammation (experimental autoimmune encephalomyelitis, EAE)1, inflammatory bowel disease (IBD)6, and type I diabetes7. Tim3 is also proposed to induce immunological tolerance7, promote Th1 apoptosis8, and regulate macrophage activation1. In addition, Tim3 is upregulated on tumorinfiltrating lymphocytes, and coblockade of Tim3 and immune checkpoint inhibitors, such as PD1, is currently being investigated in clinical trials for the treatment of cancer9. Antigen Distribution Tim3 is expressed on activated Th1 and Tc1 lymphocytes, CD11b+ macrophages, Tregs, NK cells, and mast cells. Ligand/Receptor Putative ligand on resting CD4+ lymphocytes Function May play a role in the development of immune responses and the development of Th1mediated responses PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology . Inhibitory Molecules References & Citations1. Monney, L. et al. (2002)Nature 415, 536–541. 2. Gao, X. et al. (2012) PLOS ONE 7, e30676. 3. Anderson, A. C. et al. (2007) Science 318, 1141–1143. 4. Ndhlovu, L. C. et al. (2012) Blood 119, 3734–3743. 5. Phong, B. L. et al. J. (2015) Exp. Med. 212, 2289–2304. 6. Li, X. et al. (2010) Clin. Immunol. 134, 169–177. 7. SanchezFueyo, A. et al. (2003) Nat. Immunol. 4, 1093–1101. 8. Zhu C, et al.. Nature Immunology. 2005;6:1245–1252. |