Anti-NOD1 antibody

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-2000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	NOD1
Gene ID:	10392
Uniprot ID:	NOD1_HUMAN
Immunogen Region:	290-340 aa
Specificity:	This antibody detects endogenous levels of NOD1 at Human/Mouse
Immunogen:	Synthesized peptide derived from the human NOD1 at the amino acid range 290-340

Post Translational Modifications	Palmitoylated. Palmitoylation is required for proper recruitment to the bacterial entry site and hence for proper signaling upon cognate peptidoglycan detection. Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF34 promotes proteasomal degradation and thereby negatively regulates NOD1 for instance in NF-kappa-B activation. Degraded via selective autophagy following interaction with IRGM. IRGM promotes NOD1-RIPK2 RIPosome recruitment to autophagosome membranes, promoting their SQSTM1/p62-dependent autophagic degradation.
Function	Pattern recognition receptor (PRR) that detects bacterial peptidoglycan fragments and other danger signals and thus participates in both innate and adaptive immune responses. Specifically recognizes and binds gamma-D-glutamyl-meso-diaminopimelic acid (iE-DAP), a dipeptide present in peptidoglycan of Gram-negative bacteria. Preferentially binds iE-DAP in tripeptide-containing muropeptides (MurNAc-TriDAP or TriDAP). Ligand binding triggers oligomerization that facilitates the binding and subsequent activation of the proximal adapter receptor-interacting RIPK2. Following recruitment, RIPK2 undergoes 'Met-1'- (linear) and 'Lys-63'-linked polyubiquitination by E3 ubiquitin-protein ligases XIAP, BIRC2, BIRC3 and the LUBAC complex, becoming a scaffolding protein for downstream effectors, triggering activation of the NF-kappa-B and MAP kinases signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response. Also acts as a regulator of antiviral response elicited by dsRNA and the expression of RLR pathway members by targeting IFIH1 and TRAF3 to modulate the formation of IFIH1-MAVS and TRAF3-MAVS complexes leading to increased transcription of type I IFNs. Also acts as a regulator of autophagy via its interaction with ATG16L1, possibly by recruiting ATG16L1 at the site of bacterial entry. Besides recognizing pathogens, also involved in the endoplasmic reticulum stress response: acts by sensing and binding to the cytosolic metabolite sphingosine-1-phosphate generated in response to endoplasmic reticulum stress, initiating an inflammation process that leads to activation of the NF-kappa-B and MAP kinases signaling. In addition, plays a role in insulin trafficking in beta cells in a cell-autonomous manner. Mechanistically, upon recognizing cognate ligands, NOD1 and RIPK2 localize to insulin vesicles where they recruit RAB1A to direct insulin trafficking through the cytoplasm. Isoform 3: In contrast to isoform 1, does not efficiently recognize and bind gamma-D-glutamyl-meso-diaminopimelic acid (iE-DAP) ligand.
Protein Name	Nucleotide-Binding Oligomerization Domain-Containing Protein 1 Hnod1 Caspase Recruitment Domain-Containing Protein 4
Database Links	Reactome: R-HSA-168638 Reactome: R-HSA-445989 Reactome: R-HSA-450302 Reactome: R-HSA-450321 Reactome: R-HSA-5689896 Reactome: R-HSA-9020702 Reactome: R-HSA-9705671
Cellular Localisation	Cell Membrane Lipid-Anchor Apical Cell Membrane Basolateral Cell Membrane Cytoplasm Detected In The Cytoplasm And At The Cell Membrane Following Bacterial Infection Localizes To Bacterial Entry Sites In The Cell Membrane Recruited To The Basolateral And Apical Membranes In Polarized Epithelial Cells
Alternative Antibody Names	Anti-Nucleotide-Binding Oligomerization Domain-Containing Protein 1 antibody Anti-Hnod1 antibody Anti-Caspase Recruitment Domain-Containing Protein 4 antibody Anti-NOD1 antibody Anti-CARD4 antibody

Information sourced from Uniprot.org

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