APC-Cy7 Linked Polyclonal Antibody to Alanine Aminotransferase (ALT)

Référence MBS2116516-1mL

Conditionnement : 1mL

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Alanine Aminotransferase (ALT) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2116516
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MBS2116516 is an antibody targeting ALT. Also known as APC-Cy7 Linked Polyclonal Antibody to Alanine Aminotransferase (ALT). Reactive in Human, Mouse, Rat. Host: Rabbit. More details

Product Overview

Online Datasheet in PDF
Product Name
Alanine Aminotransferase (ALT), Polyclonal Antibody
Full Product Name
APC-Cy7 Linked Polyclonal Antibody to Alanine Aminotransferase (ALT)
Product Gene Name
anti-ALT antibody
Product Synonym Names
GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1
Immunogen Sequence
Ile147~Phe 263
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
>=100ug/ml (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against ALT. It has been selected for its ability to recognize ALT in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: ALT (MBS2002664)
APC-CY7 Conjugated Antibody: ALT (MBS2116516)
Matching Pairs
APC-CY7 Conjugated Antibody: ALT (MBS2116516)
Immunogen: ALT (MBS2009133)
Molecular Weight
54,637 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (145729465..145732555). Location: 8q24.3
OMIM
138200
3D Structure
ModBase 3D Structure for P24298
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen Sequence
Ile147~Phe263
Cross Reactivity
Human, Mouse, Rat
Conjugation
APC-Cy7

Product Categories/Family for anti-ALT antibody

Enzyme & Kinase; Infection Immunity; Hepatology
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-ALT antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Other Notes

Small volumes of anti-ALT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ALT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
alanine aminotransferase
NCBI Official Synonym Full Names
glutamic-pyruvate transaminase (alanine aminotransferase)
NCBI Official Symbol
GPT
NCBI Official Synonym Symbols
AAT1; ALT1; GPT1
UniProt Protein Name
Alanine aminotransferase 1
UniProt Synonym Protein Names
Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
Protein Family
NAD--protein ADP-ribosyltransferase
UniProt Gene Name
GPT
UniProt Synonym Gene Names
AAT1; GPT1; ALT1; GPT 1
UniProt Entry Name
ALAT1_HUMAN
NCBI Protein Information
alanine aminotransferase 1; GPT 1; glutamic-alanine transaminase 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1

NCBI Summary for ALT

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]
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UniProt Comments for ALT

GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.2; Transferase

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol

Molecular Function: alanine transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid biosynthetic process; L-alanine catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ALT antibody

 Reference Product  PubMed Publications
 PGP antibody  >19 publications with ALT and PGP
 GOT2 antibody  >4 publications with ALT and GOT2
 LDHB antibody  >1 publications with ALT and LDHB

Pathways associated with anti-ALT antibody

 Products by Pathway  Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram

Diseases associated with anti-ALT antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >899 publications with ALT and Liver Diseases
 Drug Toxicity Antibodies  >606 publications with ALT and Drug Toxicity
 Neoplasms Antibodies  >455 publications with ALT and Neoplasms
 Poisoning Antibodies  >377 publications with ALT and Poisoning
 Drug-Induced Liver Injury Antibodies  >339 publications with ALT and Drug-Induced Liver Injury
 Hepatitis Antibodies  >333 publications with ALT and Hepatitis
 Necrosis Antibodies  >250 publications with ALT and Necrosis
 Cardiovascular Diseases Antibodies  >208 publications with ALT and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >199 publications with ALT and Nervous System Diseases
 Liver Neoplasms Antibodies  >163 publications with ALT and Liver Neoplasms

Organs/Tissues associated with anti-ALT antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1699 publications with ALT and Liver
 Kidney Antibodies  >327 publications with ALT and Kidney
 Heart Antibodies  >178 publications with ALT and Heart
 Lung Antibodies  >158 publications with ALT and Lung
 Brain Antibodies  >144 publications with ALT and Brain
 Skin Antibodies  >125 publications with ALT and Skin
 Intestine Antibodies  >82 publications with ALT and Intestine
 Mixed Antibodies  >73 publications with ALT and Mixed
 Stomach Antibodies  >57 publications with ALT and Stomach
 Pancreas Antibodies  >39 publications with ALT and Pancreas