AR siRNA (Human)
Référence MBS828823-30nmol
Conditionnement : 30nmol
Marque : MyBiosource
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AR siRNA – Androgen Receptor Isoform 1 (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828823MBS828823 is an siRNA targeting AR. Also known as AR siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit AR expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
AR, siRNA
Full Product Name
AR siRNA (Human)
Product Gene Name
AR sirna
Product Synonym Names
DHTR; NR3C4; Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
AR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
44,643 Da
OMIM
300068
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for AR sirna
RNA Interference (RNAi)
Other Notes
Small volumes of AR sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for AR. It may not necessarily be applicable to this product.
NCBI Official Full Name
androgen receptor isoform 1
NCBI Official Synonym Full Names
androgen receptor
NCBI Official Symbol
AR
NCBI Official Synonym Symbols
KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
UniProt Protein Name
Androgen receptor
UniProt Synonym Protein Names
Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4
Protein Family
Allatostatin
UniProt Gene Name
AR
UniProt Synonym Gene Names
DHTR; NR3C4
UniProt Entry Name
ANDR_HUMAN
NCBI Protein Information
androgen receptor
NCBI Summary for AR
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for AR
AR: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.
Protein type: Transcription factor; DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: Xq12
Cellular Component: nucleoplasm; protein complex; nuclear chromatin; cytoplasm; nucleus
Molecular Function: protein dimerization activity; ligand-dependent nuclear receptor activity; zinc ion binding; androgen binding; beta-catenin binding; transcription factor binding; androgen receptor activity; protein binding; enzyme binding; DNA binding; chromatin binding; transcription factor activity; receptor binding
Biological Process: prostate gland development; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of integrin biosynthetic process; cell proliferation; cell-cell signaling; transport; androgen receptor signaling pathway; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase III promoter; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; positive regulation of integrin biosynthetic process; cell growth; sex differentiation; positive regulation of phosphorylation
Disease: Androgen Insensitivity Syndrome; Prostate Cancer; Hypospadias 1, X-linked; Androgen Insensitivity, Partial; Spinal And Bulbar Muscular Atrophy, X-linked 1
Show more Protein type: Transcription factor; DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: Xq12
Cellular Component: nucleoplasm; protein complex; nuclear chromatin; cytoplasm; nucleus
Molecular Function: protein dimerization activity; ligand-dependent nuclear receptor activity; zinc ion binding; androgen binding; beta-catenin binding; transcription factor binding; androgen receptor activity; protein binding; enzyme binding; DNA binding; chromatin binding; transcription factor activity; receptor binding
Biological Process: prostate gland development; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of integrin biosynthetic process; cell proliferation; cell-cell signaling; transport; androgen receptor signaling pathway; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase III promoter; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; positive regulation of integrin biosynthetic process; cell growth; sex differentiation; positive regulation of phosphorylation
Disease: Androgen Insensitivity Syndrome; Prostate Cancer; Hypospadias 1, X-linked; Androgen Insensitivity, Partial; Spinal And Bulbar Muscular Atrophy, X-linked 1
Research Articles on AR
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with AR sirna
| Reference Product | PubMed Publications |
|---|---|
| CTNNB1 sirna | >735 publications with AR and CTNNB1 |
| SRC sirna | >702 publications with AR and SRC |
| TMPRSS2 sirna | >116 publications with AR and TMPRSS2 |
| NCOA1 sirna | >66 publications with AR and NCOA1 |
| NCOA2 sirna | >52 publications with AR and NCOA2 |
| KLK3 sirna | >32 publications with AR and KLK3 |
| NCOR1 sirna | >30 publications with AR and NCOR1 |
| NCOA3 sirna | >29 publications with AR and NCOA3 |
| RNF14 sirna | >17 publications with AR and RNF14 |
| HSP90AA1 sirna | >4 publications with AR and HSP90AA1 |
Pathways associated with AR sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway antibodies | Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway Diagram |
| Alpha6-Beta4 Integrin Signaling Pathway antibodies | Alpha6-Beta4 Integrin Signaling Pathway Diagram |
| Androgen Receptor Signaling Pathway antibodies | Androgen Receptor Signaling Pathway Diagram |
| Coregulation Of Androgen Receptor Activity Pathway antibodies | Coregulation Of Androgen Receptor Activity Pathway Diagram |
| FOXA1 Transcription Factor Network Pathway antibodies | FOXA1 Transcription Factor Network Pathway Diagram |
| Gene Expression Pathway antibodies | Gene Expression Pathway Diagram |
| Generic Transcription Pathway antibodies | Generic Transcription Pathway Diagram |
| IL-6 Signaling Pathway antibodies | IL-6 Signaling Pathway Diagram |
| Integrated Breast Cancer Pathway antibodies | Integrated Breast Cancer Pathway Diagram |
| Integrated Pancreatic Cancer Pathway antibodies | Integrated Pancreatic Cancer Pathway Diagram |
Diseases associated with AR sirna
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >8356 publications with AR and Nervous System Diseases |
| Inflammation Antibodies | >5754 publications with AR and Inflammation |
| Heart Diseases Antibodies | >4407 publications with AR and Heart Diseases |
| Necrosis Antibodies | >3479 publications with AR and Necrosis |
| Prostatic Neoplasms Antibodies | >3258 publications with AR and Prostatic Neoplasms |
| Kidney Diseases Antibodies | >2063 publications with AR and Kidney Diseases |
| Hypertension Antibodies | >1532 publications with AR and Hypertension |
| Breast Neoplasms Antibodies | >1416 publications with AR and Breast Neoplasms |
| Liver Diseases Antibodies | >1370 publications with AR and Liver Diseases |
| Hypertrophy Antibodies | >1292 publications with AR and Hypertrophy |
Organs/Tissues associated with AR sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Bone Antibodies | >17459 publications with AR and Bone |
| Muscle Antibodies | >13659 publications with AR and Muscle |
| Skin Antibodies | >9530 publications with AR and Skin |
| Heart Antibodies | >7012 publications with AR and Heart |
| Vascular Antibodies | >5876 publications with AR and Vascular |
| Brain Antibodies | >5526 publications with AR and Brain |
| Prostate Antibodies | >4879 publications with AR and Prostate |
| Kidney Antibodies | >3864 publications with AR and Kidney |
| Liver Antibodies | >3442 publications with AR and Liver |
| Lung Antibodies | >2712 publications with AR and Lung |