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> Aromatase/CYP19A1 Protein, Human, Recombinant (His)

Aromatase/CYP19A1 Protein, Human, Recombinant (His)

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Référence NB-64-49633-10ug

Conditionnement : 10ug

Marque : Neo Biotech

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Aromatase/CYP19A1 Protein, Human, Recombinant (His)

(Synonyms: Estrogen synthase, Cytochrome P-450AROM, Cytochrome P450 19A1, CYPXIX, CYP19A1, CYP19, CYAR, Aromatase, ARO1) Copy Product Info

Synonyms: Estrogen synthase, Cytochrome P-450AROM, Cytochrome P450 19A1, CYPXIX, CYP19A1, CYP19, CYAR, Aromatase, ARO1

Catalog No. TMPH-00956 Copy Product Info
Aromatase/CYP19A1 Protein, Human, Recombinant (His) is expressed in E. coli.
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:91%
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Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Aromatase/CYP19A1 Protein, Human, Recombinant (His) is expressed in E. coli.
Species
Human
Expression System
E. coli
TagN-6xHis
Accession NumberP11511
Amino AcidMVLEMLNPIHYNITSIVPEAMPAATMPVLLLTGLFLLVWNYEGTSSIPGPGYCMGIGPLISHGRFLWMGIGSACNYYNRVYGEFMRVWISGEETLIISKSSSMFHIMKHNHYSSRFGSKLGLQCIGMHEKGIIFNNNPELWKTTRPFFMKALSGPGLVRMVTVCAESLKTHLDRLEEVTNESGYVDVLTLLRRVMLDTSNTLFLRIPLDESAIVVKIQGYFDAWQALLIKPDIFFKISWLYKKYEKSVKDLKDAIEVLIAEKRRRISTEEKLEECMDFATELILAEKRGDLTRENVNQCILEMLIAAPDTMSVSLFFMLFLIAKHPNVEEAIIKEIQTVIGERDIKIDDIQKLKVMENFIYESMRYQPVVDLVMRKALEDDVIDGYPVKKGTNIILNIGRMHRLEFFPKPNEFTLENFAKNVPYRYFQPFGFGPRGCAGKYIAMVMMKAILVTLLRRFHVKTLQGQCVESIQKIHDLSLHPDETKNMLEMIFTPRNSDRCLEH
Construction1-503 aa
Protein Purity
> 85% as determined by SDS-PAGE.
Aromatase/CYP19A1 Protein, Human, Recombinant (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsEstrogen synthase, Cytochrome P-450AROM, Cytochrome P450 19A1, CYPXIX, CYP19A1, CYP19, CYAR, Aromatase, ARO1
Research Background
A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
Chemical Properties
Molecular Weight62.0 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Cytochrome P450AROMCytochrome P450-19A1CYP19A 1CYP 19ARO 1
Related Tags: Aromatase/CYP19A1 Protein, Human, Recombinant (His) chemical structure | Aromatase/CYP19A1 Protein, Human, Recombinant (His) molecular weight