Artocarpus integrifolia Lectin (AIA) is purified by affinity chromatography (SKU: 21510001) and has a tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. This Lectin is specific for blood group types O (+SA) and T and is classified as a galactose-specific Lectin that elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin creates a novel carbohydrate-binding site involving the N terminus of the α-chain, which accounts for its carbohydrate-binding specificity for the T-antigen disaccharide Galß1,3GalNAc. Potent inhibitors of jacalin include D-galactose, ß-Met-Gal, and 2-deoxy-α-D-galactose.

Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, leading to a colored or fluorescent product or light release as a byproduct. HRP is widely used in blotting, immunoassays, and immunohistochemistry methods.

Recommended Usage: Recommended dilutions 0.5 µg / ml in 1XPBS. Less than 0.5 µg / ml will agglutinate neuraminidase treated human erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunocytochemistry, Western blotting, ELISA/ELLA, IgG purification, Mitogenic stimulations. Glycobiology.

References

1. Jiang B, Wang X, Wang L, Lv X, Li D, Liu C, Feng Z. Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (Phaseolus vulgaris) Seeds. Polymers (Basel). 2019 May 2;11(5):785.
2. S F S Mohamad et al Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system. 2020 IOP Conf. Ser.Mater. Sci. Eng. 716 012019.
3. Ahmed H, Chatterjee BP. Further characterization and immunochemical studies on the carbohydrate specificity of jackfruit (Artocarpus integrifolia) lectin. J Biol Chem. 1989 Jun 5;264(16):9365-72.
4. Wilkinson R, Neville S. Jacalin: its binding reactivity with immunoglobulin A from various mammalian species. Vet Immunol Immunopathol. 1988 Mar;18(2):195-8.
5. T. VIJAYAKUMAR* and J. A. FORRESTER. Purification and Physico-chemical Properties of Lectins from the Jack Fruit (,4rtocarpus iutegrifolia). May 30, 1985; accepted December 9, 1985.