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> Bax (BCL2-associated X protein, Apoptosis regulator BAX, BAX protein, BCL2L4, Bcl-2-like Protein 4, Bcl2-L-4) (APC)

Bax (BCL2-associated X protein, Apoptosis regulator BAX, BAX protein, BCL2L4, Bcl-2-like Protein 4, Bcl2-L-4) (APC)

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Référence 138495-AF-APC-100ul

Conditionnement : 100ul

Marque : US Biological

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138495-AF-APC Bax (BCL2-associated X protein, Apoptosis regulator BAX, BAX protein, BCL2L4, Bcl-2-like Protein 4, Bcl2-L-4) (APC)

Clone Type
Monoclonal
Host
mouse
Source
human
Swiss Prot
Q07812, 624291
Isotype
IgG1
Grade
Affinity Purified
Applications
FC FLISA IF IHC IP WB
Crossreactivity
Hu Mk
Accession #
NP_004315
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Recognizes a protein of 21kD, identified as the Bax protein. This monoclonal antibody is highly specific to Bax and shows no cross-reaction with Bcl-2a or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, FLISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Flow Cytometry: 0.5-1ug/10e6 cells
Western Blot: 0.5-1ug/ml
Immunofluorescence: 1-2ug/ml
Immunoprecipitation: 1-2ug/500ug protein lysate
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin’s lymphoma.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 2D2|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein G affinity chromatography.|Immunogen: Synthetic peptide corresponding to aa3-16 (Cys-GSGEQPRGGGPTSS) from human Bax.|Specificity: Recognizes human Bax. Species Crossreactivity: monkey. Does not react with mouse or rat.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to aa3-16 (Cys-GSGEQPRGGGPTSS) from human Bax.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Bax. Species Crossreactivity: monkey. Does not react with mouse or rat.
References
1. Hsu YT, et. al. Journal of Biological Chemistry, 1997, 272(21):13829-34. 2. Hsu YT, et. al. PNAS, 1997, 94(8):3668-72. 3. Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92.